Inhibitory Effects of Low-Energy Pulsed Ultrasonic Stimulation on Cell Surface Protein Antigen C through Heat Shock Proteins GroEL and DnaK in Streptococcus mutans
Inhibitory Effects of Low-Energy Pulsed Ultrasonic Stimulation on Cell Surface Protein Antigen C through Heat Shock Proteins GroEL and DnaK in Streptococcus mutans
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DOI：
10.1128/aem.02230-09
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发表时间：
2010-02-01
影响因子：
4.4
通讯作者：
Ito, Koichi
中科院分区：
文献类型：
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作者：
Ishibashi, Kazuya;Shimada, Koichi;Ito, Koichi
本研究涉及使用低能量脉冲超声波作为控制牙菌斑的非破坏性光动力抗菌治疗。我们研究了低能量脉冲超声对变形链球菌的抗菌和杀菌作用及其对变形链球菌细菌细胞粘附的抑制作用。结果表明，弱的抗菌和杀菌作用。然而，超声刺激小于20分钟显着降低细菌细胞粘附。为了分析抑制作用的机制，我们检测了S.变异体超声刺激20分钟后，PAc基因和蛋白表达水平明显下降，但GTF-1表达无变化。还检测了应激反应热休克蛋白GroEL和DnaK的表达。GroEL和DnaK水平显着降低超声刺激，并在没有超声刺激的情况下，加入GroEL或DnaK抑制剂后，PAc蛋白的表达也减少。这些结果表明，在S.变形蛋白可能依赖于热休克蛋白。因此，低能量脉冲超声波通过抑制PAc蛋白和热休克蛋白的表达来降低细菌粘附，并且可以用作控制牙菌斑的光动力抗菌化学疗法。
This study concerns the use of low-energy pulsed ultrasound as nondestructive photodynamic antimicrobial therapy for controlling dental plaque. We examined the antibacterial and bactericidal effects of low-energy pulsed ultrasound on mutans streptococci and its inhibitory effects on bacterial cell adhesion of Streptococcus mutans. The results indicated weak antibacterial and bactericidal effects. However, ultrasonic stimulation for less than 20 min markedly decreased bacterial cell adhesion. To analyze the mechanism underlying the inhibitory effect, we examined cell surface protein antigen C (PAc) and glucosyltransferase I (GTF-I) expression in S. mutans. The levels of PAc gene and protein expression were markedly decreased by ultrasonic stimulation for 20 min. However, no change in GTF-I expression was observed. The expression of stress response heat shock proteins GroEL and DnaK was also examined. GroEL and DnaK levels were significantly decreased by ultrasonic stimulation, and the expression of the PAc protein was also diminished upon the addition of GroEL or DnaK inhibitors without ultrasonic stimulation. These observations suggest that the expression of the PAc protein in S. mutans may be dependent on heat shock proteins. Thus, low-energy pulsed ultrasound decreases bacterial adhesion by the inhibitory effect on the PAc protein and heat shock protein expression and may be useful as photodynamic antimicrobial chemotherapy in controlling dental plaque.