Inhibition of nuclear factor-kappa B activation attenuates hydrogen peroxide-induced cytotoxicity in human lens epithelial cells
Inhibition of nuclear factor-kappa B activation attenuates hydrogen peroxide-induced cytotoxicity in human lens epithelial cells
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DOI：
10.1136/bjo.2006.107037
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发表时间：
2007-03-01
影响因子：
4.1
通讯作者：
Ohno, Shigeaki
中科院分区：
文献类型：
--
作者：
Jin, Xue-Hai;Ohgami, Kazuhiro;Ohno, Shigeaki
目的：过氧化氢（H2 O2）是参与白内障形成的主要氧化剂。透镜上皮细胞被认为是氧化损伤的第一个部位。研究H2 O2诱导的人透镜上皮细胞（HLE）细胞毒性与核因子-κ B（NF-κ B）激活的关系。方法：HLE B-3细胞在不同浓度的H2 O2刺激下，加入或不加入NF-κ B的有效抑制剂吡咯烷二硫代氨基甲酸酯（PDTC）。采用乳酸脱氢酶细胞毒性试验检测H2 O2诱导的细胞毒性。采用Western印迹和抗p65抗体免疫细胞化学方法检测NF-κ B的易位。结果：H2 O2诱导的细胞毒性呈浓度依赖性增加。PDTC处理显著抑制H2 O2诱导的细胞毒性。H_2O_2刺激后，NF-κ B B从细胞质转位到细胞核。结论：NF-κ B信号通路在H2 O2诱导的HLE细胞损伤中起重要作用，并参与了白内障的发生。
Aims: Hydrogen peroxide (H2O2) is the major oxidant involved in cataract formation. Lens epithelial cells have been suggested to be the first site of oxidative damage. The authors investigated the relationship between H2O2-induced cytotoxicity and activation of nuclear factor kappa B (NF-kappa B) in human lens epithelial (HLE) cells.Methods: HLE B-3 cells were stimulated by various concentrations of H2O2 in the presence or absence of pyrrolidine dithiocarbamate (PDTC), a potent inhibitor of NF-kappa B. H2O2-induced cytotoxicity was measured by lactate dehydrogenase cytotoxicity assay. Translocation of NF-kappa B was examined by Western blot and immunocytochemistry using anti-p65 antibody.Results: H2O2-induced cytotoxicity increased in a concentration-dependent manner. PDTC treatment significantly suppressed the cytotoxicity induced by H2O2. After stimulated with H2O2, NF-kappa B was found translocated from cytoplasm into the nuclei. PDTC treatment also inhibited the translocation of NF-kB.Conclusions: NF-kappa B signal pathway may be important in the development of H2O2-induced damage in HLE cells that is involved in cataractogenesis.