LINK-A lncRNA promotes migration and invasion of ovarian carcinoma cells by activating TGF-β pathway
LINK-A lncRNA promotes migration and invasion of ovarian carcinoma cells by activating TGF-β pathway
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DOI：
10.1042/bsr20180936
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发表时间：
2018-10-31
影响因子：
4
通讯作者：
Xue, Min
中科院分区：
文献类型：
--
作者：
Ma, Jiezhi;Xue, Min
Introduction: LINK-A lncRNA is a well-characterized oncogenic lncRNA only in triple negative breast cancer. Our study was carried out to investigate the possible involvement of LINK-A lncRNA in ovarian carcinoma. Methods: Expression of LINK-A in ovarian biopsies and plasma of both ovarian carcinoma patients and healthy females was detected by qRT-PCR. Plasma TGF-beta 1 was detected by ELISA. Correlation between plasma LINK-A and TGF-beta 1 was analyzed by Pearson correlation analysis. Correlation between plasma LINK-A and patients' clinicopathological data was analyzed by Chi-square test. LINK-A overexpression vector was transfected into cells of human ovarian carcinoma cell lines. Cell migration and invasion were detected by Transwell migration and invasion assay. TGF-beta 1 expression was detected by Western blot. Results: We found that LINK-A and TGF-beta 1 were up-regulated in ovarian carcinoma patients than in healthy controls. Plasma levels of LINK-A were positively correlated with plasma TGF-beta 1 in ovarian carcinoma patients but not in healthy controls. Plasma levels of LINK-A were correlated with distant tumor metastasis but not tumor size. LINK-A overexpression led to up-regulated TGF-beta 1 in ovarian carcinoma cells and promoted cell migration and invasion. In contrast, TGF-beta 1 treatment showed no effects on LINK-A expression but attenuated the effects of LINK-A overexpression on cell migration and invasion. Conclusions: We conclude that LINK-A lncRNA may promote migration and invasion of ovarian carcinoma cells by activating TGF-beta pathway.
