Global analysis of the Bacillus subtilis Fur regulon and the iron starvation stimulon
Global analysis of the Bacillus subtilis Fur regulon and the iron starvation stimulon
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DOI：
10.1046/j.1365-2958.2002.03113.x
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发表时间：
2002-09-01
影响因子：
3.6
通讯作者：
Helmann, JD
中科院分区：
文献类型：
--
作者：
Baichoo, N;Wang, T;Helmann, JD
枯草芽孢杆菌铁摄取抑制蛋白（Fur）协调铁饥饿的全球转录反应。我们使用DNA微阵列来定义Fur调控子和铁饥饿刺激。我们鉴定了20个操纵子（包含39个基因），它们通过皮毛突变和铁螯合剂2,2'-二吡啶处理细胞而被抑制。这些操作子是由DNase I足迹判断的Fur调控的直接目标。对6个furr调控启动子区域lacZ报告子融合的分析表明，铁的抑制是高度选择性的。除了Fur调控子外，铁饥饿还诱导了PerR调控子的成员，并导致细胞色素的表达减少。然而，我们没有发现任何证据表明在铁限制条件下毛皮直接激活或抑制基因。尽管使用19bp Fur box共识的基因组搜索有助于识别候选的Fur调控基因，但一些与Fur box相关的基因并没有被Fur明确调控，而其他基因的调控位点与传统的Fur共识几乎没有明显的相似性。
The Bacillus subtilis ferric uptake repressor (Fur) protein coordinates a global transcriptional response to iron starvation. We have used DNA microarrays to define the Fur regulon and the iron starvation stimulon. We identify 20 operons (containing 39 genes) that are derepressed both by mutation of fur and by treatment of cells with the iron chelator 2,2'-dipyridyl. These operons are direct targets of Fur regulation as judged by DNase I footprinting. Analyses of lacZ reporter fusions to six Fur-regulated promoter regions reveal that repression is highly selective for iron. In addition to the Fur regulon, iron starvation induces members of the PerR regulon and leads to reduced expression of cytochromes. However, we did not find any evidence for genes that are directly activated by Fur or repressed by Fur under iron-limiting conditions. Although genome searches using the 19 bp Fur box consensus are useful in identifying candidate Fur-regulated genes, some genes associated with Fur boxes are not demonstrably regulated by Fur, whereas other genes are regulated from sites with little apparent similarity to the conventional Fur consensus.