HLA-DRB108:03:02 and HLA-DQB106:01:01 are associated with house dust mite-sensitive allergic rhinitis in Chinese subjects
HLA-DRB108:03:02 and HLA-DQB106:01:01 are associated with house dust mite-sensitive allergic rhinitis in Chinese subjects
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HLA-DRB108:03:02 和 HLA-DQB106:01:01 与中国受试者的屋尘螨敏感过敏性鼻炎相关
DOI：
10.1002/alr.21747
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发表时间：
2016
影响因子：
6.4
通讯作者：
Zhang Luo
中科院分区：
文献类型：
--
作者：
Zhao Yanming;Zhao Yali;Li Jingyun;Zhang Yuan;Zhang Luo
变应性鼻炎（allergic rhinitis，AR）是一种受遗传和环境因素共同影响的多因素免疫性疾病。屋尘螨（HDMs）是重要的吸入性过敏原，是AR的流行病学危险因素。类似地，人类白细胞抗原（HLA）II类基因DQB 1和DRB 1已被证明与AR相关，并在免疫应答的调节中发挥重要作用。这项工作的目的是阐明与AR在HDM敏感的汉族subjects.MethodsA共142例AR患者和184名健康受试者的HLA-II基因等位基因被招募到这个病例对照研究。AR的诊断基于病史、喉部检查、皮肤点刺试验和HMD特异性免疫球蛋白E（IgE）测定。结果在整个研究队列中共检测到HLA-DRB 1基因座的35个等位基因和HLA-DQB 1基因座的19个等位基因;等位基因DQB 1 *06：01：01的频率在所有研究队列中均高于所有研究队列。（校正p值[pc] = 0.009;比值比[OR] = 2.684; 95%置信区间[CI] = 1.482至4.994）和DRB 1 *08：03：02（pc= 0.009; OR = 3.754; 95%CI = 1.724至8.851）与健康对照组相比，HDM敏感性AR患者的AR水平显着增加，被认为是中国汉族受试者AR的一个风险因素。HDM敏感性AR组中单倍型DRB 1 *08：03-DQB 1 *06：01的频率也显著高于对照组（9.5% vs 2.4%;pc= 0.005; OR = 4.182; 95% CI = 1.870至10.285）。血清学抗原类型评估进一步证实了HLA-DRB 1 *08的频率（pc= 0.022; OR = 2.982; 95% CI = 1.46 - 6.400），DRB 1 *14的频率在HDM敏感性AR患者中显著增加，（pc= 0.030; OR = 0.340; 95%CI = 0.154至0.694）和DQB 1 *05（pc= 0.030; OR = 0.459; 95%CI = 0.269至0.764）显著降低，提示HLA-DRB 1 *08可能是一个风险因素，结论HLA-DRB 1 *08：03：02和HLA-DQB 1 *06：01：01与HDM敏感性AR相关，可能是HDM致敏的汉族人发生AR的危险因素。
BackgroundAllergic rhinitis (AR) is a multifactorial immunologic disease that is influenced by both genetic and environment factors. House dust mites (HDMs) are important inhalant aeroallergens and are an epidemiologic risk factor for AR. Similarly, the human leukocyte antigen (HLA) class II genes DQB1 and DRB1 have been shown to be associated with AR and play an important role in the regulation of the immune response. The objective of this work was to elucidate the HLA‐II gene alleles associated with AR in HDM‐sensitive Han Chinese subjects.MethodsA total of 142 patients with AR and 184 healthy subjects were recruited to this case‐control study. Diagnosis of AR was based on medical history, laryngological examination, skin‐prick tests, and HMD‐specific immunoglobulin E (IgE) assays. Genotyping of HLA‐DRB1 and HAL‐DQB1 was performed by the polymerase chain reaction sequence‐based genotyping method.ResultsA total of 35 alleles of the HLA‐DRB1 locus and 19 alleles of the HLA‐DQB1 locus were genotyped in the entire study cohort; the frequencies of alleles DQB1*06:01:01 (correctedpvalue [pc] = 0.009; odds ratio [OR] = 2.684; 95% confidence interval [CI] = 1.482 to 4.994) and DRB1*08:03:02 (pc= 0.009; OR = 3.754; 95% CI = 1.724 to 8.851) were significantly increased in HDM‐sensitive AR patients compared to healthy controls and are considered to be a risk factor for AR in Chinese Han subjects. The frequency of haplotype DRB1*08:03–DQB1*06:01 in the HDM‐sensitive AR group was also significantly higher than in the control group (9.5% vs 2.4%;pc= 0.005; OR = 4.182; 95% CI = 1.870 to 10.285). Serological antigen types assessment further demonstrated the frequency of HLA‐DRB1*08 (pc= 0.022; OR = 2.982; 95% CI = 1.46 to 6.400) to be significantly increased in HDM‐sensitive AR patients, and the frequencies of DRB1*14 (pc= 0.030; OR = 0.340; 95% CI = 0.154 to 0.694) and DQB1*05 (pc= 0.030; OR = 0.459; 95% CI = 0.269 to 0.764) to be significantly decreased, compared to healthy controls, suggesting that HLA‐DRB1*08 might be a risk factor, and DRB1*14 and DQB1*05 protective factors for HDM‐sensitive AR.ConclusionHLA‐DRB1*08:03:02 and HLA‐DQB1*06:01:01 are associated with HMD‐sensitive AR and may confer a risk for development of AR in Han Chinese subjects sensitized to HDM.