EXTRACELLULAR APPEARANCE OF CALPAIN AND CALPASTATIN IN THE SYNOVIAL-FLUID OF THE KNEE-JOINT
EXTRACELLULAR APPEARANCE OF CALPAIN AND CALPASTATIN IN THE SYNOVIAL-FLUID OF THE KNEE-JOINT
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DOI：
10.1016/0006-291x(89)92347-4
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发表时间：
1989-07-31
影响因子：
3.1
通讯作者：
MURACHI, T
中科院分区：
文献类型：
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作者：
FUKUI, I;TANAKA, K;MURACHI, T
从健康成人和一些类风湿关节炎患者的膝关节获得的无细胞滑液中证实了钙蛋白酶和钙pastatin的细胞外定位。通过DEAE-cellulose和Ultrogel AcA 34色谱柱及免疫电泳印迹分析，鉴定了calpaini和calpastatin的几个分子种。与对照组相比，患者滑液中钙蛋白酶I和钙蛋白酶II分别升高6.7倍和3.5倍。在这些患者中，我们注意到心肌的重亚单位缩短。Calpastatin在患者中也有所增加，但显示出相当广泛的碎片化。
Extracellular location of calpain and calpastatin was demonstrated in the cell-free synovial fluid obtained from the knee joint of healthy adult humans and several patients with rheumatoid arthritis. Calpains I and II and a few molecular species of calpastatin were identified by chromatographies on DEAE-cellulose and on Ultrogel AcA 34 columns as well as by immunoelectrophoretic blot analysis. Calpains I and II in the synovial fluid of the patients increased 6.7 times and 3.5 times, respectively, compared with those of the control subjects. With the patients, shortening of the heavy subunits of calpains was noted. Calpastatin also increased in the patients, but it showed rather extensive fragmentation.