Detection and Discrimination of Mycoplasma pneumoniae and Mycoplasma genitalium by the in vitro DNA Amplification
Detection and Discrimination of Mycoplasma pneumoniae and Mycoplasma genitalium by the in vitro DNA Amplification
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体外DNA扩增技术对肺炎支原体和生殖支原体的检测与鉴别
DOI：
10.1111/j.1348-0421.1992.tb01638.x
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发表时间：
1992
影响因子：
2.6
通讯作者：
Tsuguo Sasaki
中科院分区：
文献类型：
--
作者：
Y. Sasaki;M. Shintani;T. Shimada;Haruo Watanabe;Tsuguo Sasaki
根据肺炎支原体和生殖支原体的16S rRNA基因序列设计引物，建立了特异、敏感的体外DNA扩增方法，用于检测和区分这两种支原体。检测限为100个M细胞。pneumoniae和1，000个M.生殖器存在于人唾液中的其他人支原体和口腔细菌均未与这些引物显示出任何交叉反应。
By using the primers designed on the bases of the sequences of the 16S rRNA genes of Mycoplasma pneumoniae and Mycoplasma genitalium, respectively, specific and sensitive in vitro DNA amplification assay system for the detection and discrimination of these two mycoplasmas was established. The detection limit of the assay was 100 cells for M. pneumoniae and 1,000 cells for M. genitalium. Neither other human mycoplasmas nor oral bacteria existing in human saliva showed any cross‐reactions with these primers.