Microbial polyhydroxyalkanoate production from lignin by Pseudomonas putida NX-1
Microbial polyhydroxyalkanoate production from lignin by Pseudomonas putida NX-1
复制标题
恶臭假单胞菌 NX-1 从木质素生产微生物聚羟基脂肪酸酯
DOI：
10.1016/j.biortech.2020.124210
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发表时间：
2021
影响因子：
11.4
通讯作者：
Jin Mingjie
中科院分区：
文献类型：
--
作者：
Xu Zhaoxian;Xu Meilin;Cai Chenggu;Chen Sitong;Jin Mingjie
生物方法在木质素的有价化中起着重要的作用，但这一领域的许多问题仍不清楚。本论文基于基因组序列技术，对恶臭假单胞菌NX-1木质素分解酶进行了系统的研究。特别是对一种染料脱色过氧化物酶进行了系统的异源表达、酶纯化和酶学性质研究，表明它对合成染料和木质素衍生的芳香族化合物都具有活性。此外，对聚羟基烷酸生物合成的完整途径进行了注释，并对聚羟基烷酸的生物合成能力进行了研究。实验证实，PutidaNX-1以木质素为唯一碳源。采用气相色谱-质谱法、凝胶渗透色谱、差示扫描量热法和热重分析等方法，对葡萄糖和木质素衍生芳香族聚羟基烷酸酯的单体组成、相对分子质量和热性能进行了综合测定。结果表明，由葡萄糖和木质素衍生的芳香族化合物制备的聚羟基烷酸酯的物理性质相似，木质素可以在不影响其质量的情况下作为聚羟基烷酸酯生产的替代原料。
Biological approaches play an important role in lignin valorization, whereas many issues in this area remain unclear. Herein, ligninolytic enzymes inPseudomonas putidaNX-1 were systematically unraveled based on genome sequence technology. Particularly, a dye-decolorizing peroxidase was systematically studied by heterologous expression, enzyme purification, and enzymatic characterization, which suggested it possessed activities on both synthetic dyes and lignin-derived aromatics. Moreover, a complete pathway for polyhydroxyalkanoate biosynthesis was annotated, and the polyhydroxyalkanoate biosynthesis capability ofP. putidaNX-1 was experimentally confirmed with lignin as the sole carbon source. Furthermore, the monomer compositions, molecular weights, and thermal properties of polyhydroxyalkanoate from glucose and lignin-derived aromatics were comprehensively determined by gas chromatography-mass spectrometry, gel permeation chromatography, differential scanning calorimetry, and thermogravimetric analysis. The results indicated that physical properties of polyhydroxyalkanoate prepared from glucose and lignin-derived aromatics were similar, which suggested lignin could be an alternative feedstock for polyhydroxyalkanoate production without compromising its quality.