MYOSATELLITE CELLS OF CYPRINUS-CARPIO (TELEOSTEI) INVITRO - ISOLATION, RECOGNITION AND DIFFERENTIATION
MYOSATELLITE CELLS OF CYPRINUS-CARPIO (TELEOSTEI) INVITRO - ISOLATION, RECOGNITION AND DIFFERENTIATION
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DOI：
10.1007/bf00329450
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发表时间：
1990-07-01
影响因子：
3.6
通讯作者：
OSSE, JWM
中科院分区：
文献类型：
--
作者：
KOUMANS, JTM;AKSTER, HA;OSSE, JWM
我们开发了一种从鲤鱼白色外轴肌中分离和纯化肌卫星细胞的方法。通过形态、超微结构、含有肌原纤维的多核肌管的形成以及结蛋白的免疫细胞化学演示来鉴定解离的肌卫星细胞。结蛋白和 5-溴-2''-脱氧尿苷 (BrdU) 分别用于鉴定终末分化和增殖的肌卫星细胞。从标准长度 5 厘米的鲤鱼中分离出来的肌卫星细胞的体外行为与哺乳动物和鸟类的肌卫星细胞的描述不同。没有观察到肌卫星细胞的大量增殖。大多数细胞在铺板后 17 小时分化（结蛋白阳性，BrdU 阴性），无论使用何种培养基。这表明所研究的标准长度为 5 厘米的鲤鱼白色外轴肌含有肌卫星细胞亚群，这些肌卫星细胞处于不同的分化阶段。
We have developed a method for the dissociation and purification of myosatellite cells from white epaxial muscle of carp. The dissociated myosatellite cells were identified by their morphology, their ultrastructure, the formation of multinucleated myotubes containing myofibrils and the immunocytochemical demonstration of desmin. Desmin and 5-bromo-2''-deoxyuridine (BrdU) were used to identify terminally differentiated and proliferating myosatellite cells, respectively. The in vitro behavior of myosatellite cells dissociated from carp of 5 cm standard length differed from that described for myosatellite cells of mammals and birds. No substantial proliferation of the myosatellite cells could be observed. Most cells were differentiated (desmin-positive, BrdU-negative) 17 h after plating, regardless of the medium used. This indicates that the investigated white epaxial muscle of carp of 5 cm standard length contains subpopulations of myosatellite cells, arrested at various stages of differentiation.