ANCYLOSTOMA-CANINUM - METALLOPROTEASE RELEASE COINCIDES WITH ACTIVATION OF INFECTIVE LARVAE IN-VITRO
ANCYLOSTOMA-CANINUM - METALLOPROTEASE RELEASE COINCIDES WITH ACTIVATION OF INFECTIVE LARVAE IN-VITRO
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DOI：
10.1006/expr.1995.1025
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发表时间：
1995-03-01
影响因子：
2.1
通讯作者：
HOTEZ, PJ
中科院分区：
文献类型：
--
作者：
HAWDON, JM;JONES, BF;HOTEZ, PJ
已知几种线虫的感染阶段会在排泄/分泌产物中释放蛋白酶。这些酶被认为促进了组织的侵袭，尽管缺乏直接的证据。在这些调查中，我们采用了一种检测方法，将恢复进食作为感染过程中早期事件的标志。当犬钩吻线虫三期感染幼虫在体外被刺激摄食时，它们释放出约50,000和90,000分子量的蛋白酶，通过底物凝胶电泳法测定。锌螯合剂1，10-菲咯啉对酶有抑制作用，而非螯合异构体4，7-菲咯啉对酶无抑制作用，说明该酶的蛋白水解力依赖于锌。这两种化合物都能抑制体外摄食，尽管1，10-菲咯啉的抑制作用依赖于锌，而4，7-菲咯啉的抑制作用不依赖于锌。与食性启动相关的特定的蛋白水解酶的释放表明，金属蛋白水解酶在自由生活阶段向寄生阶段的转变中起着不可或缺的作用。(C)1995年学术出版社。
Infective stages of several nematode parasites are known to release proteases in excretory/secretory products. These enzymes are believed to facilitate tissue invasion, although direct evidence is lacking. For these investigations, we employed an assay that uses the resumption of feeding as a marker for early events in the infectious process. When Ancylostoma caninum third-stage infective larvae are stimulated to feed in vitro, they release proteases of approximately 50,000 and 90,000 molecular weight, as determined by substrate gel electrophoresis. The enzymes are inhibited by the zinc chelator 1,10-phenanthroline, but not by the nonchelating isomer 4,7-phenanthroline, indicating that the proteolytic activity is zinc-dependent. Both compounds inhibit in vitro feeding, although inhibition by 1,10-phenanthroline is zinc-dependent, whereas inhibition by 4,7-phenanthroline is zinc-independent. The specific release of proteases associated with the initiation of feeding suggests that the metalloprotease serves an integral function in the transition of the free-living stage to parasitism. (C) 1995 Academic Press, Inc.