IDENTIFICATION OF A VIRULENCE-ASSOCIATED ANTIGEN OF TOXOPLASMA-GONDII BY USE OF A MOUSE MONOCLONAL-ANTIBODY
IDENTIFICATION OF A VIRULENCE-ASSOCIATED ANTIGEN OF TOXOPLASMA-GONDII BY USE OF A MOUSE MONOCLONAL-ANTIBODY
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DOI：
10.1128/iai.59.12.4511-4516.1991
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发表时间：
1991-12-01
影响因子：
3.1
通讯作者：
HEESEMANN, J
中科院分区：
文献类型：
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作者：
GROSS, U;MULLER, WA;HEESEMANN, J
制备了一种抗刚地弓形虫RH株小鼠致死性单克隆抗体。在永久性巨噬细胞培养物中培养的毒性和无毒弓形虫分离物的速殖子检测了与该抗体反应性的差异。通过向NMRI小鼠注射不同数量的速殖子并观察动物感染或死亡的迹象，量化了这些弓形虫分离株的毒力。该单克隆抗体鉴定出小鼠致死菌株BK和RH表达的23-kDa抗原，而该抗原未在小鼠低毒力菌株中检测到，这些菌株均为来自欧洲的临床分离株。利用免疫印迹（免疫印迹）、免疫荧光和免疫电镜，我们将23-kDa抗原定位到膜室。根据这些结果，我们认为这个23-kDa抗原是一个毒株毒力的标记，可以作为弓形虫毒力分类的基础。
A monoclonal antibody generated against the mouse-lethal RH strain of Toxoplasma gondii was developed. Tachyzoites of virulent and avirulent T. gondii isolates grown in permanent macrophage cell cultures were examined for differences in reactivity with this antibody. Virulence of these Toxoplasma isolates was quantified by injecting different numbers of tachyzoites into NMRI mice and observing the animals for signs of infection or death. The monoclonal antibody identified a 23-kDa antigen expressed by the mouse-lethal strains BK and RH, whereas this antigen was not detected in low-mouse-virulent strains, which were all clinical isolates from Europe. Using Western blot (immunoblot), immunofluorescence, and immunoelectron microscopy, we localized the 23-kDa antigen to the membrane compartment. From these results, we suggest that this 23-kDa antigen is a marker of strain virulence upon which a virulence classification of T. gondii may be based.