Dendritic organization of sensory input to cortical neurons in vivo
Dendritic organization of sensory input to cortical neurons in vivo
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DOI：
10.1038/nature08947
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发表时间：
2010-04-29
期刊：
影响因子：
64.8
通讯作者：
Konnerth, Arthur
中科院分区：
文献类型：
--
作者：
Jia, Hongbo;Rochefort, Nathalie L.;Konnerth, Arthur
在感觉皮层区域，神经元被调谐到特定的刺激特征。例如，在视觉皮层中，许多神经元主要对移动物体的偏好方向做出反应。然而，皮层神经元接收突触输入以产生其输出放电模式的特征仍不清楚。在这里，我们报告了一种新的方法，用于可视化和功能映射的感觉输入皮层神经元树突在体内。通过将高速双光子成像与电生理记录相结合，我们确定了与定向特异性突触输入相对应的局部阈下钙信号。我们发现，即使是具有相同取向偏好的输入也广泛分布在整个树突树中。同时，不同取向偏好的输入被穿插，使得相邻的树突片段被调谐到不同的取向。因此，定向调谐神经元可以通过整合空间分布的突触输入编码来计算其特征放电模式。
In sensory cortex regions, neurons are tuned to specific stimulus features. For example, in the visual cortex, many neurons fire predominantly in response to moving objects of a preferred orientation. However, the characteristics of the synaptic input that cortical neurons receive to generate their output firing pattern remain unclear. Here we report a novel approach for the visualization and functional mapping of sensory inputs to the dendrites of cortical neurons in vivo. By combining high-speed two-photon imaging with electrophysiological recordings, we identify local subthreshold calcium signals that correspond to orientation-specific synaptic inputs. We find that even inputs that share the same orientation preference are widely distributed throughout the dendritic tree. At the same time, inputs of different orientation preference are interspersed, so that adjacent dendritic segments are tuned to distinct orientations. Thus, orientation-tuned neurons can compute their characteristic firing pattern by integrating spatially distributed synaptic inputs coding for multiple stimulus orientations.