NUCLEOTIDE-SEQUENCES OF THE MESSENGER-RNAS ENCODING THE VESICULAR STOMATITIS VIRUS-G AND VIRUS-M PROTEINS DETERMINED FROM CDNA CLONES CONTAINING THE COMPLETE CODING REGIONS
NUCLEOTIDE-SEQUENCES OF THE MESSENGER-RNAS ENCODING THE VESICULAR STOMATITIS VIRUS-G AND VIRUS-M PROTEINS DETERMINED FROM CDNA CLONES CONTAINING THE COMPLETE CODING REGIONS
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DOI：
10.1128/jvi.39.2.519-528.1981
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发表时间：
1981-01-01
影响因子：
5.4
通讯作者：
GALLIONE, CJ
中科院分区：
文献类型：
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作者：
ROSE, JK;GALLIONE, CJ
编码糖蛋白（G）和基质蛋白（M）的水泡性口炎病毒mRNA的完整核苷酸序列是从含有每个mRNA的完整编码序列的c[互补]DNA克隆中确定的。G蛋白mRNA是1665个核苷酸长，不包括polyA，并且编码包括16个氨基酸的信号肽的511个氨基酸的蛋白质。G蛋白含有2个大的疏水结构域，一个位于信号肽中，另一个位于靠近COOH末端的跨膜片段中。预测在氨基酸残基178和335处有两个糖基化位点。这些位点的位置与报告的G合成期间添加2种寡糖的时间密切对应，表明一旦适当的天冬酰胺残基穿过[仓鼠肾BHK细胞]的粗面内质网膜，就会发生糖基化。编码水泡性口炎病毒M蛋白的mRNA是831个核苷酸长，不包括polyA，并编码229个氨基酸的蛋白质。预测的M蛋白序列不包含任何长的疏水或非极性结构域，可能会促进膜协会。该蛋白质富含碱性氨基酸，并含有高度碱性的氨基末端结构域。近全长cDNA克隆的建设的细节。
The complete nucleotide sequences of the vesicular stomatitis virus mRNA encoding the glycoprotein (G) and the matrix protein (M) were determined from c[complementary]DNA clones that contain the complete coding sequences from each mRNA. The G protein mRNA is 1665 nucleotides long, excluding polyA, and encodes a protein of 511 amino acids including a signal peptide of 16 amino acids. G protein contains 2 large hydrophobic domains, one in the signal peptide and the other in the transmembrane segment near the COOH terminus. Two sites of glycosylation are predicted at amino acid residues 178 and 335. The close correspondence of the positions of these sites with the reported timing of the addition of the 2 oligosaccharides during synthesis of G suggests that glycosylation occurs as soon as the appropriate asparagine residues traverse the membrane of the rough endoplasmic reticulum [of hamster kidney BHK cells]. The mRNA encoding the vesicular stomatitis virus M protein is 831 nucleotides long, excluding polyA, and encodes a protein of 229 amino acids. The predicted M protein sequence does not contain any long hydrophobic or nonpolar domains that might promote membrane association. The protein is rich in basic amino acids and contains a highly basic amino terminal domain. Details of construction of the nearly full-length cDNA clones are presented.