SEVERE ACUTE GRAFT-VERSUS-HOST DISEASE BY HLA-DPB1 DISPARITY IN RECOMBINANT FAMILY OF BONE-MARROW TRANSPLANTATION BETWEEN SEROLOGICALLY HLA-IDENTICAL SIBLINGS - AN APPLICATION OF THE POLYMERASE CHAIN REACTION-RESTRICTION FRAGMENT LENGTH POLYMORPHISM METHOD
SEVERE ACUTE GRAFT-VERSUS-HOST DISEASE BY HLA-DPB1 DISPARITY IN RECOMBINANT FAMILY OF BONE-MARROW TRANSPLANTATION BETWEEN SEROLOGICALLY HLA-IDENTICAL SIBLINGS - AN APPLICATION OF THE POLYMERASE CHAIN REACTION-RESTRICTION FRAGMENT LENGTH POLYMORPHISM METHOD
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DOI：
10.1016/0198-8859(91)90089-r
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发表时间：
1991-12-01
期刊：
影响因子：
2.7
通讯作者：
TSUJI, K
中科院分区：
文献类型：
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作者：
NOMURA, N;OTA, M;TSUJI, K
HLA-DP抗原的匹配在骨髓移植（BMT）中起着关键作用，这主要是由于DP分型的致敏淋巴细胞试验（PLT）方法的困难。我们以前报道了一种有效的技术，HLA II类基因分型，通过消化聚合酶链反应（PCR）扩增的基因与限制性片段长度多态性（RFLP）内切酶（PCR-RFLP方法）。应用PCR-RFLP方法对46例HLA血清学完全相合同胞骨髓移植受者和相应供者的DNA进行了DP分型。在46例病例中，5例（10.9%）为DP基因不匹配（每个减数分裂中DR-DQ和DP亚区之间的重组频率至少为2.7%），这为观察仅在DP抗原中观察到的差异对BMT的影响提供了重要机会。四个DP不匹配的BMT病例中有三个发生了严重的急性移植物抗宿主病，这表明DP不匹配在BMT中发挥了重要作用。
It has remained to be established that matching of the HLA-DP antigen plays a key role in bone marrow transplantation (BMT), mainly due to the difficulty of the primed lymphocyte test (PLT) method for DP typing. We previously reported an efficient technique for HLA class II genotyping, by digestion of polymerase chain reaction (PCR)-amplified genes with restriction fragment length polymorphisms (RFLP) endonucleases (PCR-RFLP method). DNAs from 46 recipients and corresponding donors in serologically HLA-identical sibling-BMT cases were DP typed by this PCR-RFLP method. Of the 46 cases, five (10.9%) were genetically DP mismatched (recombinant frequency between the DR-DQ and DP subregions was at least 2.7% per meiosis), providing an important opportunity to look at the effect of the disparity only seen in the DP antigen on BMT. Three of the four DP-mismatched BMT cases that could be evaluated developed severe acute graft-versus-host disease, suggesting that DP disparity played an important role in BMT.