Vital fluorescent stains for detection of stress in Pythium ultimum and Rhizoctonia solani challenged with viscosinamide from Pseudomonas fluorescens DR54
Vital fluorescent stains for detection of stress in Pythium ultimum and Rhizoctonia solani challenged with viscosinamide from Pseudomonas fluorescens DR54
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用于检测来自荧光假单胞菌 DR54 的粘酰胺的终极腐霉和立枯丝核菌应激检测的重要荧光染色
DOI：
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Jan Sørensen
中科院分区：
文献类型：
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作者：
C. Thrane;S. Olsson;Tommy Harder Nielsen;Jan Sørensen
来自荧光假单胞菌 DR54 的粘酰胺对植物病原微真菌终极腐霉和立枯丝核菌具有生物防治作用。这些真菌和其他四种真菌的生理变化证据是通过使用卡尔科弗鲁白、尼罗红、Syto 13、3,3'-二庚基氧羰花青、5-（和-6）-羧基 FDA 和 5-（和-6）-羧基 SNARF-1、乙酰氧基甲基、乙酸酯进行活体荧光染色获得的。根据对固体琼脂培养基上生长的真菌和卡尔科弗洛白染色的研究，所有分离株对粘酰胺处理的反应是分枝增加，有时包括菌丝肿胀和间隔增加。此外，染色模式表明酯酶活性、细胞内 pH 值以及线粒体组织和活性的变化。几种真菌显示出尼罗红染色减少，表明细胞膜或细胞壁的疏水性普遍降低。相比之下，只有腐霉属。可用核酸染色剂 Syto 13 进行染色，并显示出细胞核对粘酰胺的反应发生的变化。最后，结果表明 Calcoflor White、Nile red 和 Syto 13 也可用于利用土壤微观世界研究真菌胁迫。该研究表明，重要荧光染色剂的组合适合对细菌抗生素等引起的真菌应激进行详细研究。
Viscosinamide from Pseudomonas fluorescens DR54 exerts a biocontrol effect on the plant pathogenic microfungi Pythium ultimum and Rhizoctonia solani. Evidence for physiological changes in these and four other fungi was obtained by vital fluorescent staining with Calcofluor white, Nile red, Syto 13, 3,3′-diheptyloxacarbocyanine, 5-(and-6)-carboxy FDA and 5-(and-6)-carboxy SNARF-1, acetoxymethyl, acetate. Based on studies of the fungi grown on solid agar medium and Calcofluor white staining, all isolates responded to viscosinamide treatment by an increased branching, sometimes including hyphal swelling and an increased septation. Further, the staining pattern indicated changes in the esterase activity, intracellular pH and mitochondrial organization and activity. Several of the fungi showed a reduced Nile red staining indicating a general decrease in hydrophobicity of cellular membranes or cell walls. In contrast, only Pythium spp. were stainable with the nucleic acid stain Syto 13 and showed changes in the nuclei in response to viscosinamide. Finally, it was shown that Calcofluor white, Nile red and Syto 13 could also be used to study fungal stress in situ using soil microcosms. The study demonstrated that combinations of vital fluorescent stains are suitable for detailed studies of fungal stress as induced by, e.g., bacterial antibiotics.
粗糙脉孢菌对热休克的呼吸反应。
影响因子：
2.1
作者：
Habel,D;Plesofsky-Vig,N;Brambl,R
通讯作者：
Brambl,R