Expression profiling during adipocyte differentiation of 3T3-L1 fibroblasts
Expression profiling during adipocyte differentiation of 3T3-L1 fibroblasts
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DOI：
10.1016/s0378-1119(02)01017-x
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发表时间：
2002-10-16
期刊：
影响因子：
3.5
通讯作者：
Stevens, GJ
中科院分区：
文献类型：
--
作者：
Jessen, BA;Stevens, GJ
3T3-L1细胞系是一种成熟且常用的体外模型，用于评估脂肪细胞分化。经过几天的过程，融合的3T3-L1细胞可以在致脂混合物的存在下转化为脂肪细胞。在脂肪细胞向成熟脂肪细胞分化过程中的三个时间点（24小时、4天和1周），通过DNA微阵列检测基因表达的变化。脂肪细胞转化影响了几个功能类别的基因。此外，发现七个基因在所有三个时间点通过脂肪细胞转化通常改变了5倍或更多。脂肪细胞分化可诱导脂钙蛋白2、接触珠蛋白、血清淀粉样蛋白A3、硬脂酰辅酶a去饱和酶和11β -羟基类固醇脱氢酶1，同时抑制肌动蛋白α 2和前胶原VIII α 1。对这些基因和通路调控的进一步研究将有助于进一步了解脂肪细胞分化的生化途径，并可能为肥胖和其他代谢性疾病的治疗找到新的治疗靶点。(C) 2002 Elsevier Science B.V.版权所有
The 3T3-L1 cell line is a well-established and commonly used in vitro model to assess adipocyte differentiation. Over the course of several days confluent 3T3-L1 cells can be converted to adipocytes in the presence of an adipogenic cocktail. Changes in gene expression were measured by DNA microarrays at three time points (24 h, 4 days, and I week) during the course of differentiation from preadipocytes to mature adipocytes. Several functional categories of genes were affected by adipocyte conversion. In addition, seven genes were found to be commonly altered by 5-fold or more by adipocyte conversion at all three time points. Lipocalin 2, haptoglobin, serum amyloid A3, stearoyl-CoA desaturase, and 11beta-hydroxysteroid dehydrogenase 1 were induced while actin alpha2 and procollagen VIII alpha1 were suppressed by adipocyte differentiation. Further study of the regulation of these genes and pathways will lead to an increased understanding of the biochemical pathways involved in adipocyte differentiation and possibly to the identification of new therapeutic targets for treatment of obesity and other metabolic diseases. (C) 2002 Elsevier Science B.V. All rights reserved.