Stacking of Crick Wobble pair and Watson-Crick pair: stability rules of G-U pairs at ends of helical stems in tRNAs and the relation to codon-anticodon Wobble interaction.
Stacking of Crick Wobble pair and Watson-Crick pair: stability rules of G-U pairs at ends of helical stems in tRNAs and the relation to codon-anticodon Wobble interaction.
复制标题
Crick Wobble 对和 Watson-Crick 对的堆叠：tRNA 中螺旋茎末端 G-U 对的稳定性规则以及与密码子-反密码子 Wobble 相互作用的关系。
DOI：
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发表时间：
1978
影响因子：
14.9
通讯作者：
M. Sundaralingam
中科院分区：
文献类型：
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作者：
H. Mizuno;M. Sundaralingam
发现螺旋末端的非互补G-U碱基对的发生受堆积相互作用的支配。通常，在沃森-克里克碱基对的5 '侧具有G的G-U对与沃森-克里克碱基对的堆叠重叠比沃森-克里克碱基对的3'侧的G-U对显著更大。预期前一种排列更稳定，实际上在已知的转移RNA分子中观察到32次中的29次。根据这一规则，密码子-反密码子相互作用中的主要摆动碱基对G-U或I-U在反密码子的5 '侧具有G或I。类似地，在起始tRNA中，G是密码子的第一个字母（5 '侧）的情况下也遵循这一规则。在U位于反密码子摆动位置的情况下，它通常在C（5）处被取代，也可能具有2-硫代基团，并且根据其修饰，它可以读取一到四个密码子。反密码子摆动位置的G可以识别以U或C结尾的两个密码子，并且G的修饰（除非是I）不会改变其阅读特性。
The occurrence of the noncomplementary G-U base pair at the end of a helix is found to be governed by stacking interactions. As a rule, a G-U pair with G on the 5'-side of a Watson-Crick base pair exhibits strikingly greater stacking overlap with the Watson-Crick base pair than a G-U pair on the 3'-side of a Watson-Crick base pair. The former arrangement is expected to be more stable and indeed is observed 29 times out of 32 in the known transfer RNA molecules. In accordance with this rule, the major wobble base pairs G-U or I-U in codon-anticodon interactions have G or I on the 5'-side of the anticodon. Similarly, in initiator tRNAs, this rule is obeyed where now the G is the first letter of the codon (5'-side). In the situation where U is in the wobble position of the anticodon, it is usually substituted at C(5) andmay also have a 2-thio group and it can read one to four codons depending on its modifications. A G at the wobble position of the anticodon can recognize the two codons ending with U or C and modification of G (unless it is I) does not change its reading properties.