Molecular characterisation and expression profiling of the ENO1 gene in the ovarian follicle of the Sichuan white goose
Molecular characterisation and expression profiling of the ENO1 gene in the ovarian follicle of the Sichuan white goose
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DOI：
10.1007/s11033-014-3039-3
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发表时间：
2014-04-01
影响因子：
2.8
通讯作者：
Ma, Rong
中科院分区：
文献类型：
--
作者：
Kang, Bo;Jiang, Dong Mei;Ma, Rong
ENO1基因编码一种多功能酶，已被确定为糖酵解途径的关键组成部分。我们前期的研究表明，ENO1基因在产蛋鹅卵巢中的表达水平高于产蛋前期鹅。然而，ENO1基因在鹅组织和卵巢卵泡中的分子特征和表达谱仍有待确定。本研究克隆了四川白鹅ENO1基因全长1,445个碱基，并对其进行了序列分析。ENO1基因全长1,305个碱基，编码434个氨基酸，相对分子质量为47.27 kDa。ENO1在所有受检组织中的表达以脾最高，胸肌最低。ENO1在肾脏、肝脏、肾上腺和视网膜均有高表达。随着卵泡生长的增加，ENO1基因的表达从小的白色卵泡到F5开始下降，然后在F4表达急剧增加，然后从F3到F1表达逐渐下降。此外，在外阴后卵泡(POF)中，ENO1基因的表达水平从POF1到POF4逐渐降低。综上所述，ENO1转录本在四川白鹅的不同组织中广泛分布，但ENO1的表达具有组织特异性。此外，卵巢卵泡ENO1的表达谱结果表明，ENO1可能在卵泡发育过程中发挥着重要的双重作用，其中ENO1既是一种糖酵解酶，也是一种介导细胞凋亡的酶。
The ENO1 gene encodes a multifunctional enzyme that has been identified as a key component of the glycolytic pathway. Our previous studies demonstrated that ENO1 gene expression was higher in the ovaries of laying geese compared with prelaying geese. However, the molecular characterisation and expression profiling of the ENO1 gene in geese tissues and ovarian follicles remain to be determined. In this study, ENO1 cDNA (1,445 bp long) of the Sichuan white goose was cloned and characterised. The ORF of ENO1 cDNA is 1,305 bp in length and encodes a 434 amino acid protein with a molecular weight of 47.27 kDa. ENO1 expression in all of the examined tissues was the highest in spleen and the lowest in breast muscle. High expression of ENO1 appeared in the kidney, liver, adrenal gland, and retina. With increasing follicle growth, ENO1 gene expression began to decrease from the small white follicle to F5, which was followed by a sharp increase in expression in F4 and then a gradual decrease in expression from F3 to F1. Furthermore, in the postovulatory follicles (POF), the levels of ENO1 gene expression decreased gradually from POF1 to POF4. In conclusion, the ENO1 transcript was widely distributed in various tissues of the Sichuan white goose, but ENO1 expression was tissue-specific. Furthermore, the results of the ENO1 expression profiling of ovarian follicles suggest that ENO1 may play an important dual role in the progress of follicular development, where ENO1 acts as a glycolytic enzyme and also mediates apoptosis.