Semantide- and chemotaxonomy-based analyses of some problematic phenotypic clusters of slowly growing mycobacteria, a cooperative study of the international working group on mycobacterial taxonomy
Semantide- and chemotaxonomy-based analyses of some problematic phenotypic clusters of slowly growing mycobacteria, a cooperative study of the international working group on mycobacterial taxonomy
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DOI：
10.1099/00207713-46-1-280
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发表时间：
1996-01-01
期刊：
影响因子：
--
通讯作者：
Johnson, R
中科院分区：
文献类型：
--
作者：
Wayne, LG;Good, RC;Johnson, R
在以前的合作数值分类研究缓慢增长的分枝杆菌，国际工作组分枝杆菌分类描述了一些菌株的分类地位是模糊的。对这些生物中的66种的DNA、RNA和蛋白质进行了一项新的研究，以将它们的特性与表型聚类行为相关联;这项研究的结果允许将所研究的51种菌株分配给已知物种。用于表征semantides的方法包括核苷酸测序和通过亲和结合技术（包括全DNA-DNA杂交、探针杂交和抗体结合）评估semantides相关性水平。有良好的表型和化学分类集群和semantide分析确定的生物体组之间的总体协议。我们的研究结果支持这样的结论，我们应该继续依靠多相分类，以提供令人满意的系统分辨率属分枝杆菌的成员。我们没有发现单一的16 S rRNA菌株间核苷酸序列差异值，明确界定物种边界。DNA-DNA杂交仍然是黄金标准，但需要共同的资源，使那些不准备使用这种技术的实验室能够进行DNA杂交分析。我们研究的一个大型新簇对应于最近描述的物种间质分枝杆菌，一种在表型上类似于非病原体戈登分枝杆菌的病原体。我们还鉴定了似乎代表胞内分枝杆菌核糖体的菌株，其不与目前用于鉴定该种的商业诊断探针反应。其他分支或集群组成的菌株太少，无法决定它们的分类地位。
During previous cooperative numerical taxonomic studies of slowly growing mycobacteria, the International Working Group on Mycobacterial Taxonomy described a number of strains whose taxonomic status was ambiguous. A new study of DNA, RNA, and proteins from 66 of these organisms was performed to correlate their properties with phenotypic clustering behavior; the results of this study permitted 51 of the strains studied to be assigned to known species. The methods used to characterize the semantides included nucleotide sequencing and assessment of levels of semantide relatedness by affinity binding techniques, including whole DNA-DNA hybridization, probe hybridization, and antibody binding. There was good overall agreement between the phenotypic and chemotaxonomic clusters and the groups of organisms identified by semantide analyses. Our results supported the conclusion that we should continue to rely on polyphasic taxonomy to provide satisfactory systematic resolution of members of the genus Mycobacterium. We identified no single 16S rRNA interstrain nucleotide sequence difference value that unequivocally defined species boundaries. DNA-DNA hybridization remains the gold standard, but common resources are needed to permit DNA DNA hybridization analyses to be made available to laboratories that are not prepared to use this technology. One of the large novel clusters which we studied corresponds to the recently described species Mycobacterium interjectum, a pathogen that resembles the nonpathogen Mycobacterium gordonae phenotypically. We also identified strains that appear to represent ribovars of Mycobacterium intracellulare which do not react with the commercial diagnostic probes that are currently used for identification of this species. Other branches or clusters consisted of too few strains to permit a decision about their taxonomic status to be made.