Sodium Fluoride Affects Proliferation and Apoptosis Through Insulin-Like Growth Factor I Receptor in Primary Cultured Mouse Osteoblasts
Sodium Fluoride Affects Proliferation and Apoptosis Through Insulin-Like Growth Factor I Receptor in Primary Cultured Mouse Osteoblasts
复制标题
氟化钠通过胰岛素样生长因子i受体影响原代培养的小鼠成骨细胞的增殖和凋亡
DOI：
10.1007/s12011-011-9059-0
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发表时间：
2011-12-01
影响因子：
3.9
通讯作者：
Su, Jingliang
中科院分区：
文献类型：
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作者：
Ren, Gaixian;Ferreri, Miro;Su, Jingliang
胰岛素样生长因子-I受体（IGF-IR）在维持成骨细胞增殖和凋亡中起重要作用。然而，关于氟化钠补充对原代成骨细胞培养物介导IGF-IR表达的影响的细节仍然不为人所知。本研究以小鼠成骨细胞为研究对象，探讨氟化钠对成骨细胞增殖和凋亡的影响;用IGF-IR小干扰RNA处理细胞（或不处理），随后分为对照组和六个实验组，分别暴露于不同浓度的NaF（10（-6），10（-5），10（-4），10（-3），5 × 10（-3），和10（-2）mol/L）。特别是，我们研究了细胞增殖，细胞凋亡，IGF-IR信使RNA和蛋白质表达水平的各种细胞群。总之，我们的研究结果表明，氟化钠的管理影响IGF-IR在小鼠成骨细胞的表达，有助于氟诱导的增殖和凋亡。
Insulin-like growth factor-I receptor (IGF-IR) is important for maintaining proliferation and apoptosis in osteoblasts. However, the details about the effect of sodium fluoride supplementation on primary osteoblast cultures on mediating IGF-IR expression are still not well-known. In this study, we used mouse osteoblasts to examine the impact of sodium fluoride on the proliferation and apoptosis; the cells were treated with IGF-IR small interfering RNA (or left untreated) and subsequently divided into a control group and six experimental groups, which were exposed to different concentrations of NaF (10(-6), 10(-5), 10(-4), 10(-3), 5 x 10(-3), and 10(-2) mol/L) for analysis at 48 h. In particular, we examined cell proliferation, apoptosis, IGF-IR messenger RNA, and protein expression levels of the various cell groups. In summary, our findings suggest that the administration of NaF affects the expression of IGF-IR in mouse osteoblasts, contributing to the proliferation and apoptosis induced by fluoride.