An RNA pyrophosphohydrolase triggers 5'-exonucleolytic degradation of mRNA in Bacillus subtilis.
An RNA pyrophosphohydrolase triggers 5'-exonucleolytic degradation of mRNA in Bacillus subtilis.
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DOI：
10.1016/j.molcel.2011.07.023
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发表时间：
2011-09-16
期刊：
影响因子：
16
通讯作者：
Belasco JG
中科院分区：
文献类型：
--
作者：
Richards J;Liu Q;Pellegrini O;Celesnik H;Yao S;Bechhofer DH;Condon C;Belasco JG
在大肠杆菌中，RNA降解通常始于5′-末端三磷酸转化为一磷酸，为RNase E的内部切割创造更好的底物。值得注意的是，这种关键核酸内切酶的同源物不存在于许多细菌物种中，例如枯草芽孢杆菌和各种病原体。在这里，我们报告的降解的初级转录本在B。然而，枯草芽孢杆菌可以通过类似的过程触发以产生短寿命的单磷酸化中间体。就像它的E。coli对应物，即B.枯草杆菌RNA焦磷酸水解酶催化这一事件，是一种偏好不成对5′端的Nuvillin蛋白。然而，在B. subtilis这种修饰使转录物暴露于RNase J的快速5′-核酸外切降解，而在E.大肠杆菌中存在，但在大多数缺乏RNase E的细菌中存在。该途径与真核细胞中去腺苷化mRNA的降解机制非常相似，解释了此类细菌中5 '末端茎环的稳定影响。
In Escherichia coli, RNA degradation often begins with conversion of the 5′-terminal triphosphate to a monophosphate, creating a better substrate for internal cleavage by RNase E. Remarkably, no homologue of this key endonuclease is present in many bacterial species, such as Bacillus subtilis and various pathogens. Here we report that the degradation of primary transcripts in B. subtilis can nevertheless be triggered by an analogous process to generate a short-lived, monophosphorylated intermediate. Like its E. coli counterpart, the B. subtilis RNA pyrophosphohydrolase that catalyzes this event is a Nudix protein that prefers unpaired 5′ ends. However, in B. subtilis this modification exposes transcripts to rapid 5′-exonucleolytic degradation by RNase J, which is absent in E. coli but present in most bacteria lacking RNase E. This pathway, which closely resembles the mechanism by which deadenylated mRNA is degraded in eukaryotic cells, explains the stabilizing influence of 5′-terminal stem-loops in such bacteria.
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