Development of in situ and ex situ seed baiting techniques to detect mycorrhizal fungi from terrestrial orchid habitats
Development of in situ and ex situ seed baiting techniques to detect mycorrhizal fungi from terrestrial orchid habitats
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DOI：
10.1017/s0953756203008463
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发表时间：
2003-10-01
影响因子：
--
通讯作者：
Sivasithamparam, K
中科院分区：
文献类型：
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作者：
Brundrett, MC;Scade, A;Sivasithamparam, K
提出了一种创新的异位真菌诱饵方法，使用从现场收集的土壤，可以同时检测多个陆生兰花的菌根真菌。结果表明，枯落物和表土中的粗粒有机质（> 2mm）是这些真菌最重要的接种库。同时介绍了一种新的原位种子诱饵方法，使用多室包，同时评估不同兰花物种在土壤中的发芽。这些原位和异位的方法进行了比较，使用种子的兰花属Monadenia，Microtis，Caladenia，Pterostylis和Diuris，使用城市Banksia林地网站的杂草覆盖率高或低。这两种种子诱饵方法检测这些兰花兼容的真菌，但常见的兰花发芽比那些不常见的现场。发芽率没有显着影响杂草覆盖，即使成年兰花是罕见的高杂草覆盖的地区。这两种新的种子饵诱方法的效率和适用性因使用情况而异。然而，异位方法允许发芽的时间过程中观察，导致更多的原球茎的生产和促进菌根真菌的分离。这些技术提供了有价值的新工具，检测兼容的菌根真菌，以协助兰花的研究和保护。
An innovative ex situ fungal baiting method using soil collected from field sites which allows the simultaneous detection of mycorrhizal fungi for multiple terrestrial orchids is presented. This method demonstrated that coarse organic matter (> 2 mm) in the litter and topsoil was the most important reservoir of inoculum of these fungi. A new in situ seed baiting method using multi-chambered packets to simultaneously assess germination for different orchid species within soil is also introduced. These in situ and ex situ methods are compared using seed of orchids in the genera Monadenia, Microtis, Caladenia, Pterostylis and Diuris, using urban Banksia woodland sites with high or low weed cover. Both these seed baiting methods detected compatible fungi for these orchids, but common orchids germinated more frequently than those which were uncommon at the field sites. Germination rates were not significantly affected by weed cover even though adult orchids were rare in areas with high weed cover. The two new seed baiting methods vary in efficiency and applicability depending on the situation where they are used. However, the ex situ method allowed the time-course of germination to be observed, resulting in the production of more protocorms and facilitation of the isolation of mycorrhizal fungi. These techniques provide valuable new tools for detection of compatible mycorrhizal fungi to assist orchid research and conservation.