Variety-Selective Rhizospheric Activation, Uptake, and Subcellular Distribution of Perfluorooctanesulfonate (PFOS) in Lettuce (Lactuca sativa L.)
Variety-Selective Rhizospheric Activation, Uptake, and Subcellular Distribution of Perfluorooctanesulfonate (PFOS) in Lettuce (Lactuca sativa L.)
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生菜 (Lactuca sativa L.) 中全氟辛烷磺酸 (PFOS) 的品种选择性根际激活、吸收和亚细胞分布
DOI：
10.1021/acs.est.1c01175
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发表时间：
2021
影响因子：
11.4
通讯作者：
Li Qing X.
中科院分区：
文献类型：
--
作者：
Yu Peng-Fei;Li Yan-Wen;Zou Long-Jun;Liu Bai-Lin;Xiang Lei;Zhao Hai-Ming;Li Hui;Cai Quan-Ying;Hou Xue-Wen;Mo Ce-Hui;Wong Ming Hung;Li Qing X.
全氟辛烷磺酸（PFOS）是一种在农作物中具有累积性的新出现的持久性有机污染物，对人类健康构成严重威胁。已经确定了全氟辛烷磺酸累积量较低的莴苣品种（低累积作物品种，LACV），但相关机制仍未得到解决。在这里，根际激活，吸收，转运，和分区的全氟辛烷磺酸在LACV与高积累作物品种（HACV）的比较，根际形态，转运，和亚细胞分布的全氟辛烷磺酸。根系分泌物中的溶解性有机质对根际土壤中PFOS的解吸有促进作用，但LACV中的作用弱于HACV。植物根系对PFOS的吸收是通过一种转运蛋白介导的被动过程来控制的，在该过程中，水通道蛋白和快速型阴离子通道的低活性通过LACV根系中低水平的PIP基因（PIP 1 - 1和PIP 2 -2）和ALMT基因（ALMT 10和ALMT 13）的表达水平来校正。根细胞壁和营养体中的全氟辛烷磺酸比例越高，LACV中的根到茎的运输量就越低。LACV科普全氟辛烷磺酸对芽细胞毒性的能力比HACV差，因为全氟辛烷磺酸在叶绿体中的比例较高，但在液泡中的比例较低。我们的研究结果提供了新的见解PFOS在生菜中的积累和进一步了解LACV的多进程机制。
Perfluorooctanesulfonate (PFOS) as an accumulative emerging persistent organic pollutant in crops poses severe threats to human health. Lettuce varieties that accumulate a lower amount of PFOS (low-accumulating crop variety, LACV) have been identified, but the regarding mechanisms remain unsolved. Here, rhizospheric activation, uptake, translocation, and compartmentalization of PFOS in LACV were investigated in comparison with those of high-accumulating crop variety (HACV) in terms of rhizospheric forms, transporters, and subcellular distributions of PFOS. The enhanced PFOS desorption from the rhizosphere soils by dissolved organic matter from root exudates was observed with weaker effect in LACV than in HACV. PFOS root uptake was controlled by a transporter-mediated passive process in which low activities of aquaporins and rapid-type anion channels were corrected with low expression levels ofPIPs(PIP1–1andPIP2–2) andALMTs(ALMT10andALMT13) genes in LACV roots. Higher PFOS proportions in root cell walls and trophoplasts caused lower root-to-shoot transport in LACV. The ability to cope with PFOS toxicity to shoot cells was poorer in LACV relative to HACV since PFOS proportions were higher in chloroplasts but lower in vacuoles. Our findings provide novel insights into PFOS accumulation in lettuce and further understanding of multiprocess mechanisms of LACV.