Microbial production and characterization of poly-3-hydroxybutyrate by Neptunomonas antarctica.
Microbial production and characterization of poly-3-hydroxybutyrate by Neptunomonas antarctica.
复制标题
南极海王单胞菌聚-3-羟基丁酸酯的微生物生产和表征
作者：
Liu XJ;Zhang J;Hong PH;Li ZJ
考虑到可生物降解聚合物聚3-羟基丁酸酯（PHB）在工业上的应用价值，研究了海洋细菌南极海王星单胞菌（Neptunomonasanapartica）对聚3-羟基丁酸酯的富集能力。提取的聚合物经核磁共振分析证实为聚羟基丁酸。在以天然海水为培养基成分的摇瓶培养中，聚羟基丁酸的产量可达2.12g/L，产率为0.18g聚羟基丁酸/g果糖。在人工海水存在下，发酵液中的聚羟基丁酸的效价和产率分别达到2.13 g/L和0.13 g聚羟基丁酸/g果糖。当果糖耗尽时，积累的聚合物逐渐减少，表明细胞内的PHB被N降解。南极洲天然海水中生成的聚羟基丁酸的重均分子量为2.4 × 105 g/mol，数均分子量为1.7 × 105 g/mol。我们的结果突出了N.用于以海水作为营养源生产聚羟基丁酸的厌氧微生物。
Considering the industrial interest of biodegradable polymer poly-3-hydroxybutyrate (PHB), the marine bacteria Neptunomonas antarctica was studied for its ability to accumulate PHB. The extracted polymer was confirmed to be PHB by nuclear magnetic resonance analysis. In shake flask cultures using natural seawater as medium components, PHB was produced up to 2.12 g/L with a yield of 0.18 g PHB/g fructose. In the presence of artificial seawater, the PHB titer and yield reached 2.13 g/L and 0.13 g PHB/g fructose, respectively. The accumulated polymer gradually decreased when fructose was exhausted, indicating that intracellular PHB was degraded by N. antarctica. The weight-average and number-average molecular weights of PHB produced within natural seawater were 2.4 × 10 5 g/mol and 1.7 × 10 5 g/mol, respectively. Our results highlight the potential of N. antarctica for PHB production with seawater as a nutrient source.