Size measurement of Daphnia pulex using low-coherence Gabor digital holography
Size measurement of Daphnia pulex using low-coherence Gabor digital holography
复制标题
使用低相干 Gabor 数字全息术测量水蚤的尺寸
DOI：
10.1007/s10043-019-00558-8
复制
发表时间：
2019
期刊：
影响因子：
1.2
通讯作者：
Hayasaki Y
中科院分区：
文献类型：
--
作者：
Sunayama K;Miyakawa H;Hayasaki Y
我们测量了水蚤（D. pulex）的大小，这是一种用于检查毒性的指示生物，在自由游泳和无压力的条件下，通过数字全息术（DH）的后聚焦能力成为可能。这种环境测量是DH的一个新应用。该实现是本研究的重点，应适用于非无菌和不稳定机械情况下的实际现场使用。通过反复试验，我们得出了最简单的干涉仪配置，使用可承受振动的单光束线、用于减少相干噪声的低相干光源以及单次图像捕获。进行了基于DH和图像处理的自动尺寸测量。本研究的独创性在于，通过将结果与使用普通光学显微镜观察和图像处理软件进行的传统人工肉眼测量的结果进行比较，实现了定量的尺寸测量和性能评估。该估计还使我们能够确定 DH 测量的图像处理中的适当参数。
We measured the size ofDaphnia pulex(D. pulex), which is an indicator organism used for examining toxicity, under freely-swimming and stress-free conditions, made possible by the post-focusing ability of digital holography (DH). This environmental measurement is a new application of DH. This implementation, which is a key point of this research, should be applicable to real on-site use in non-sterile and unstable mechanical situations. Through trial and error, we arrived at the simplest interferometer configuration using a single beam line that can withstand vibrations, a low-coherence light source for reducing coherent noise, and single-shot image capturing. Automatic size measurement based on DH with image processing was performed. The originality of this research is the quantitative size measurement and performance estimation achieved by comparing the results with those from conventional manual eye measurement using ordinary optical microscope observation and image processing software. The estimation also allowed us to determine the proper parameters in the image processing for DH measurement.