Decreased interleukin(IL)-35 Expression is Associated with Active Intraocular Inflammation in Vogt-Koyanagi-Harada (VKH) Disease
Decreased interleukin(IL)-35 Expression is Associated with Active Intraocular Inflammation in Vogt-Koyanagi-Harada (VKH) Disease
复制标题
白细胞介素 (IL)-35 表达减少与 Vogt-小柳原田 (VKH) 病的活动性眼内炎症相关。
DOI：
10.1080/09273948.2018.1433306
复制
发表时间：
2019-05-19
影响因子：
3.3
通讯作者：
Li, Hong
中科院分区：
文献类型：
--
作者：
Hu, Jianping;Qin, Yirong;Li, Hong
目的：近年来研究表明IL-35在自身免疫性疾病中具有保护作用。在这项研究中，我们探讨了IL-35在Vogt-Koyanagi-Harada（VKH）病发病机制中的作用。方法：采用Real-Time PCR方法检测IL-35/EBI 3和IL-35/P35 mRNA水平。ELISA法检测血清IL-35水平。PBMC和单核细胞衍生的DC在有或没有IL-35的情况下培养，并通过ELISA测试上清液中IL-17、IL-10、IFN-γ、IL-6、TNF-α和IL-1 β的浓度。结果：活动期VKH患者血清IL-35水平降低。活动期VKH患者PBMC中IL-35/EBI 3和IL-35/P35两个亚基的mRNA表达也降低。IL-35显著抑制IFN-γ和IL-17表达，诱导PBMC产生IL-10，并抑制单核细胞衍生的DC产生IL-6。结论：IL-35表达降低可能参与了VKH病的发病过程。
Purpose: Recent studies have reported that IL-35 has a protective effect in autoimmune disease. In this study, we explored the role of IL-35 in the pathogenesis of Vogt-Koyanagi-Harada (VKH) disease. Methods: The IL-35/EBI3 and IL-35/P35 mRNA level was assayed by Real-Time PCR. The level of IL-35 in serum was detected by ELISA. PBMCs and monocyte-derived DCs were cultured with or without IL-35 and the concentration of IL-17, IL-10, IFN-gamma, IL-6, TNF-alpha, and IL-1 beta in supernatants was tested by ELISA. Results: The serum level of IL-35 is reduced in active VKH patients. The mRNA expression of the two subunits IL-35/EBI3 and IL-35/P35 in PBMCs from patients with active VKH was also decreased. IL-35 significantly inhibited IFN-gamma and IL-17 expression and induced IL-10 production by PBMCs and inhibited IL-6 production by monocyte-derived DCs. Conclusion: The current study suggests that a decreased IL-35 expression may be involved in the pathogenesis of VKH disease.