BACILLUS-SUBTILIS POSSESSES A 2ND DETERMINANT WITH EXTENSIVE SEQUENCE SIMILARITY TO THE ESCHERICHIA-COLI-MREB MORPHOGENE
BACILLUS-SUBTILIS POSSESSES A 2ND DETERMINANT WITH EXTENSIVE SEQUENCE SIMILARITY TO THE ESCHERICHIA-COLI-MREB MORPHOGENE
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DOI：
10.1128/jb.177.3.765-773.1995
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发表时间：
1995-02-01
影响因子：
3.2
通讯作者：
STEWART, GC
中科院分区：
文献类型：
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作者：
ABHAYAWARDHANE, Y;STEWART, GC
A、Decatur、B、Kunkel和R在染色体图谱上的302度处鉴定出与枯草芽孢杆菌的mre B形态基因具有实质性序列相似性的基因。Losick（哈佛大学;个人通讯）.我们的表征表明，该决定簇的蛋白产物（称为mreB样的mbl）与B的Mre B蛋白在序列上有55%和53%相同。该蛋白质与鉴定为来自蜡状芽孢杆菌的Mre B的蛋白质具有86%的同一性，表明蜡状芽孢杆菌的B蛋白质实际上是Mbl。mbl的插入失活表明该基因对细胞活力或孢子形成不是必需的。携带突变mbl等位基因的细胞显示生长速率降低和细胞形态改变。细胞出现肿胀，并经常扭曲，基因间抑制突变，恢复生长速度到一个近似正常的水平出现在突变群体。第二个位点突变，命名为som-1，通过转导定位到染色体的hisA-mbl区域。
A gene with substantial sequence similarity to the mreB morphogene of Bacillus subtilis has been identified at 302 degrees on the chromosomal map by A, Decatur, B, Kunkel, and R. Losick (Harvard University; personal communication). Our characterization has revealed that the protein product of this determinant (termed mbl for mreB-like) is 55 and 53% identical in sequence to the MreB proteins of B. subtilis and Escherichia coli, respectively, The protein is 86% identical to a protein identified as MreB from Bacillus cereus, suggesting that the B, cereus protein is actually Mbl. Insertional inactivation of mbl indicated that this gene is not essential for cell viability or sporulation, Cells bearing mutant mbl alleles display a decreased growth rate and an altered cellular morphology. The cells appear bloated and are frequently twisted, Intergenic suppressor mutations which restore the growth rate to an approximately normal level arise within the mutant population. A second site mutation, designated som-1, was mapped to the hisA-mbl region of the chromosome by transduction.