Rapid Typing of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae Isolates by Use of SpectraCell RA
Rapid Typing of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae Isolates by Use of SpectraCell RA
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DOI：
10.1128/jcm.05423-11
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发表时间：
2012-04-01
影响因子：
9.4
通讯作者：
Maquelin, Kees
中科院分区：
文献类型：
--
作者：
Willemse-Erix, Diana;Bakker-Schut, Tom;Maquelin, Kees
肠杆菌科细菌是医院感染和社区获得性感染的重要病原菌。特别是，具有广谱β-内酰胺酶的菌株越来越多地在卫生保健环境中引起问题。快速和可靠的分型系统是确定传播的关键工具，以便能够采取有针对性的感染控制措施。在本研究中，我们使用SpectraCell RA细菌菌株分析仪（River Diagnostics）评价了拉曼光谱分析（RA）对多重耐药大肠埃希菌和肺炎克雷伯菌分离株进行分型的性能。96个不相关的分离株的分析表明，RA产生高度可重复的光谱，并表现出歧视的权力，是脉冲场凝胶电泳相媲美。此外，获得了三个临床分离株的充分结果。RA能够区分与爆发相关的分离株与不参与爆发或传播的分离株。此外，发现RA方法识别克隆，与超广谱β-内酰胺酶类型无关。因此，RA是一种较好的大肠杆菌分型技术。埃希菌和肺炎分离株。结合高重现性，速度和易用性，这种技术可能会发挥重要作用，在监测这些重要的医院内物种的流行病学。
Enterobacteriaceae are important pathogens of both nosocomial and community-acquired infections. In particular, strains with broad-spectrum beta-lactamases increasingly cause problems in health care settings. Rapid and reliable typing systems are key tools to identify transmission, so that targeted infection control measures can be taken. In this study, we evaluated the performance of Raman spectroscopic analysis (RA) for the typing of multiresistant Escherichia coli and Klebsiella pneumoniae isolates using the SpectraCell RA bacterial strain analyzer (River Diagnostics). Analysis of 96 unrelated isolates revealed that RA generated highly reproducible spectra and exhibited a discriminatory power that is comparable to pulsed-field gel electrophoresis. Furthermore, adequate results were obtained for three collections of clinical isolates. RA was able to discriminate outbreak-related isolates from isolates that were not involved in an outbreak or transmission. Furthermore, it was found that the RA approach recognized clones, irrespective of the extended-spectrum beta-lactamase type. It can be concluded that RA is a suitable typing technique for E. coli and K. pneumoniae isolates. Combining high reproducibility, speed, and ease-of-use, this technique may play an important role in monitoring the epidemiology of these important nosocomial species.