Effect of chondroitin sulfate proteoglycans on neuronal cell adhesion, spreading and neurite growth in culture.
Effect of chondroitin sulfate proteoglycans on neuronal cell adhesion, spreading and neurite growth in culture.
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硫酸软骨素蛋白聚糖对培养物中神经元细胞粘附、扩散和神经突生长的影响。
DOI：
10.4103/1673-5374.226398
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发表时间：
2018-03
影响因子：
6.1
通讯作者：
Yu P
中科院分区：
文献类型：
--
作者：
Jin J;Tilve S;Huang Z;Zhou L;Geller HM;Yu P
作为中枢神经系统细胞外基质(ECM)的主要成分之一，硫酸软骨素蛋白多糖(CSPGs)长期以来一直被认为是富含在胶质瘢痕中的抑制物，可以阻止损伤后轴突的再生。尽管许多研究表明CSPGs在体外使用不同类型的神经元抑制轴突生长，但CSPGs抑制轴突生长的机制仍然知之甚少。采用小脑颗粒神经元(CGN)培养方法，研究了不同浓度的固定化和可溶性CSPG对神经元生长的影响，包括细胞黏附、铺展和突起生长。随着CSPGs浓度的升高，轴突长度减少，细胞密度降低，细胞聚集体形成增多。可溶性CSPGs对轴突生长也有抑制作用，但诱导细胞聚集所需的浓度高于包被的CSPGs。我们还发现，CSPG上的生长锥大小显著减小，神经细胞的铺展受到抑制，这归因于CSPG对板脂伸展的抑制。干涉反射显微镜(IRM)进一步证实了CSPG对神经元黏附的影响，直接证明CGN和大脑皮质神经元对CSPG底物的黏附更加松散。这些数据表明，CSPG除了对轴突生长有影响外，还对细胞的黏附和扩散有影响。
As one major component of extracellular matrix (ECM) in the central nervous system, chondroitin sulfate proteoglycans (CSPGs) have long been known as inhibitors enriched in the glial scar that prevent axon regeneration after injury. Although many studies have shown that CSPGs inhibited neurite outgrowth in vitro using different types of neurons, the mechanism by which CSPGs inhibit axonal growth remains poorly understood. Using cerebellar granule neuron (CGN) culture, in this study, we evaluated the effects of different concentrations of both immobilized and soluble CSPGs on neuronal growth, including cell adhesion, spreading and neurite growth. Neurite length decreased while CSPGs concentration arised, meanwhile, a decrease in cell density accompanied by an increase in cell aggregates formation was observed. Soluble CSPGs also showed an inhibition on neurite outgrowth, but it required a higher concentration to induce cell aggregates formation than coated CSPGs. We also found that growth cone size was significantly reduced on CSPGs and neuronal cell spreading was restrained by CSPGs, attributing to an inhibition on lamellipodial extension. The effect of CSPGs on neuron adhesion was further evidenced by interference reflection microscopy (IRM) which directly demonstrated that both CGNs and cerebral cortical neurons were more loosely adherent to a CSPG substrate. These data demonstrate that CSPGs have an effect on cell adhesion and spreading in addition to neurite outgrowth.
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影响因子：
4.2
作者：
GUNDERSEN, RW
通讯作者：
GUNDERSEN, RW
DOI：
10.1083/jcb.118.2.431
发表时间：
1992-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Iida J;Skubitz AP;Furcht LT;Wayner EA;McCarthy JB
通讯作者：
McCarthy JB
影响因子：
5.3
作者：
Aricescu, AR;McKinnell, IW;Stoker, AW
通讯作者：
Stoker, AW
影响因子：
5.3
作者：
Kerstein PC;Nichol RH IV;Gomez TM
通讯作者：
Gomez TM
影响因子：
25
作者：
通讯作者：
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