Expression of colorectal neoplasia differentially expressed in anaplastic thyroid carcinoma and its effect on cancer cell proliferation.
Expression of colorectal neoplasia differentially expressed in anaplastic thyroid carcinoma and its effect on cancer cell proliferation.
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结直肠肿瘤在甲状腺未分化癌中差异表达及其对癌细胞增殖的影响
DOI：
10.21037/atm-22-945
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发表时间：
2022-04
影响因子：
--
通讯作者：
Qiao, Hong
中科院分区：
文献类型：
--
作者：
Du, Lili;Zhao, Qingsong;Li, Jingjing;Wang, Mingli;Qiao, Hong
关键词：
背景：间变性甲状腺癌（ATC）在人类癌症中发病率很高。结直肠肿瘤差异表达（CRNDE）在常见肿瘤中高表达，因此是抗肿瘤治疗的潜在分子靶点。然而，CRNDE在ATC中的作用尚不清楚。方法利用基因表达综合数据库（Gene Expression Omnibus， GEO）筛选长链非编码RNA （lncRNA）在ATC组织中的差异表达。利用癌症基因组图谱（Cancer Genome Atlas， TCGA）数据库分析CRNDE在甲状腺癌（THCA）组织中的表达及其对患者预后的影响。采用实时荧光定量PCR （Quantitative real-time PCR, qRT-PCR）检测CRNDE在肿瘤及对照组织中的表达水平。采用TCGA RNA测序（RNA-seq）数据分析，探讨CRNDE在THCA中的生物学功能。选择CRNDE低表达和高表达的ATC细胞系进行CRNDE siRNA转染，检测各组细胞的增殖情况。结果GEO和TCGA数据库分析结果显示，CRNDE在ATC组织中高表达，与THCA患者预后不良有关。CRNDE在ATC细胞系ARO（人甲状腺癌细胞系）中的表达量较高，而在sw579中的表达量较低。因此，我们选择ARO和sw579转染CRNDE小干扰RNA （siRNA）。与阴性对照（si-NC）相比，CRNDE在si-CRNDE-1、si-CRNDE-2、si-CRNDE-3中的表达降低，表明抑制作用明显增强，细胞增殖能力降低，细胞周期停留在G0/G1期。最后，我们发现si-CRNDE-1和si-CRNDE-2的wnt3a、β-catenin和cyclinD1蛋白表达显著降低。结论CRNDE在ATC组织中的高表达可能通过调控Wnt/β-catenin信号通路促进ATC细胞的增殖。CRNDE可能是治疗ATC的潜在分子靶点。
Background The incidence of anaplastic thyroid cancer (ATC) is high among human cancers. Colorectal neoplasia differentially expressed (CRNDE) is highly expressed in common tumors, and is therefore a potential molecular target for anti-tumor therapy. However, the function of CRNDE in ATC remains elusive. Methods The Gene Expression Omnibus (GEO) database was used to screen the differential expression of long-noncoding RNA (lncRNA) in ATC tissues. The Cancer Genome Atlas (TCGA) database was used to analyze the expression of CRNDE in thyroid cancer (THCA) tissues and its impact on patient prognosis. Quantitative real-time PCR (qRT-PCR) was used to determine the expression level of CRNDE in tumor and control tissues. The biological function of CRNDE in THCA was explored using TCGA RNA sequencing (RNA-seq) data analysis. ATC cell lines with low and high CRNDE expression were selected for CRNDE siRNA transfection, and the proliferation of cells was detected in each group. Results The GEO and TCGA databases analysis results showed that CRNDE was highly expressed in ATC tissues, which is related to the poor prognosis of THCA patients. Also, the expression of CRNDE in the ATC cell line, ARO (human thyroid cancer cell line), was relatively high, while the expression in sw579 is relatively low. Therefore, ARO and sw579 were chosen for CRNDE small interfering RNA (siRNA) transfection. Compared with negative control (si-NC), the expression of CRNDE in si-CRNDE-1, si-CRNDE-2, and si-CRNDE-3 was reduced, indicating that the inhibitory effect was significantly enhanced and the cell proliferation ability was reduced, and the cell cycle is arrested in the G0/G1 phase. Finally, it was found that the wnt3a, β-catenin, and cyclinD1 protein expressions of si-CRNDE-1 and si-CRNDE-2 were significantly reduced. Conclusions The high expression of CRNDE in ATC tissues may promote the proliferation of ATC cells by regulating the Wnt/β-catenin signaling pathway. CRNDE may be a potential molecular target for the treatment of ATC.
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α1-肾上腺素能受体拮抗剂对趋化因子（C-X-C 矩阵）受体 4 和非典型趋化因子受体 3 的部分激动活性。
影响因子：
3.7
作者：
Gao X;Abdelkarim H;Albee LJ;Volkman BF;Gaponenko V;Majetschak M
通讯作者：
Majetschak M
DOI：
10.1007/978-3-030-38266-7_1
发表时间：
2020-01-01
期刊：
PROTEOSTASIS AND DISEASE: FROM BASIC MECHANISMS TO CLINICS
影响因子：
--
作者：
Perez-Benavente, Beatriz;Fathinajafabadi Nasresfahani, Alihamze;Farras, Rosa
通讯作者：
Farras, Rosa
影响因子：
7.5
作者：
Jing, Hongyu;Xia, Huan;Lv, Xiaohong
通讯作者：
Lv, Xiaohong
甲状腺未分化癌主调节因子的综合生物信息学分析
影响因子：
--
作者：
Pan, Zongfu;Li, Lu;Huang, Ping
通讯作者：
Huang, Ping
影响因子：
3.6
作者：
Sebastian, Jomon;Rathinasamy, Krishnan
通讯作者：
Rathinasamy, Krishnan