The roles of Rhodobacter sphaeroides copper chaperones PCu(A)C and Sco (PrrC) in the assembly of the copper centers of the aa(3)-type and the cbb(3)-type cytochrome c oxidases.
The roles of Rhodobacter sphaeroides copper chaperones PCu(A)C and Sco (PrrC) in the assembly of the copper centers of the aa(3)-type and the cbb(3)-type cytochrome c oxidases.
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球形红杆菌铜伴侣 PCu(A)C 和 Sco (PrrC) 在 aa(3) 型和 cbb(3) 型细胞色素 c 氧化酶铜中心组装中的作用。
DOI：
10.1016/j.bbabio.2012.01.003
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Hosler,JonathanP
中科院分区：
文献类型：
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作者：
Thompson,AudieK;Gray,Jimmy;Liu,Aimin;Hosler,JonathanP
α蛋白酶Rhodobacter sphaeroides在好氧生长过程中在其细胞质膜上积累两种细胞色素c氧化酶（CcO）：一种是aa 3型CcO，含有一个CuA中心和一个CuB中心;另一种是cbb 3型CcO，含有CuB中心但不含CuA中心。R的周质中有三个铜分子伴侣。sphaeroides、PCuAC、PrrC（Sco）和Cox11。Cox 11是组装aa 3型CuB而不是cbb 3型CcO所必需的。PrrC与线粒体Sco 1同源; Sco蛋白参与线粒体和细菌中的CuA组装，并与cbb 3型CcO的CuB组装有关。PCuAC存在于许多细菌中，但不是线粒体。嗜热栖热菌的PCuAC在体外可使CuA中心金属化，但其在体内的功能尚未被探索。在此，我们对R.已在缺乏PCuAC、PrrC或两者的菌株中检查了类球孢菌。任一分子伴侣的缺乏强烈降低了在低浓度Cu 2+中生长的细胞中两种CCO的积累。PrrC的缺乏比PCuAC的缺乏具有更大的影响，并且PCuAC似乎在PrrC的上游起作用。纯化的aa 3型CcO的分析表明，PrrC有一个更大的影响，其组装的Cu A比PCuAC，和两个分子伴侣有一个较小的，但显着的影响，其组装的Cu B，即使Cox 11的存在。PCuAC和PrrC的细胞作用的情况被认为是。结果是最一致的作用PrrC在捕获和交付铜的CuA的aa 3型CcO和CuB的cbb 3型CcO，而PCuAC的主要作用可能是捕获和交付铜的PrrC和Cox 11。这篇文章是题为：呼吸酶复合物的生物发生/组装特刊的一部分。
The α proteobacter Rhodobacter sphaeroides accumulates two cytochrome c oxidases (CcO) in its cytoplasmic membrane during aerobic growth: a mitochondrial-like aa3-type CcO containing a di-copper CuAcenter and mono-copper CuB, plus a cbb3-type CcO that contains CuBbut lacks CuA. Three copper chaperones are located in the periplasm of R. sphaeroides, PCuAC, PrrC (Sco) and Cox11. Cox11 is required to assemble CuBof the aa3-type but not the cbb3-type CcO. PrrC is homologous to mitochondrial Sco1; Sco proteins are implicated in CuAassembly in mitochondria and bacteria, and with CuBassembly of the cbb3-type CcO. PCuAC is present in many bacteria, but not mitochondria. PCuAC of Thermus thermophilus metallates a CuAcenter in vitro, but its in vivo function has not been explored. Here, the extent of copper center assembly in the aa3- and cbb3-type CcOs of R. sphaeroides has been examined in strains lacking PCuAC, PrrC, or both. The absence of either chaperone strongly lowers the accumulation of both CcOs in the cells grown in low concentrations of Cu2+. The absence of PrrC has a greater effect than the absence of PCuAC and PCuAC appears to function upstream of PrrC. Analysis of purified aa3-type CcO shows that PrrC has a greater effect on the assembly of its CuAthan does PCuAC, and both chaperones have a lesser but significant effect on the assembly of its CuBeven though Cox11 is present. Scenarios for the cellular roles of PCuAC and PrrC are considered. The results are most consistent with a role for PrrC in the capture and delivery of copper to CuAof the aa3-type CcO and to CuBof the cbb3-type CcO, while the predominant role of PCuAC may be to capture and deliver copper to PrrC and Cox11. This article is part of a Special Issue entitled: Biogenesis/Assembly of Respiratory Enzyme Complexes.