Overexpression of rice NAC gene SNAC1 in ramie improves drought and salt tolerance
Overexpression of rice NAC gene SNAC1 in ramie improves drought and salt tolerance
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DOI：
10.1007/s10725-014-9991-z
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发表时间：
2015-06-01
影响因子：
4.2
通讯作者：
Peng, Dingxiang
中科院分区：
文献类型：
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作者：
An, Xia;Liao, Yiwen;Peng, Dingxiang
当传统育种不可行时，转基因技术可能是改良作物的最佳途径。苎麻就是这样一种植物，因为杂合子使育种工作复杂化。本研究首次报道了成功获得耐旱耐盐的转基因苎麻植株。本研究首次以苎麻叶片中脉为外植体，通过农杆菌介导法将逆境应答基因SNAC 1（STRESS-RESPONSIVE NAC 1）导入苎麻。通过对转基因植株和野生型植株的表型和产量的比较，发现SNAC 1的过表达显著提高了幼苗和纤维成熟期的耐旱性和耐盐性。SNAC 1过表达的苎麻植株在快速生长阶段对干旱和盐胁迫的耐受性也显着提高。在干旱和盐胁迫下，转基因植株在快速生长期的光合速率和相对含水量均高于野生型植株。本研究表明可以通过基因工程手段提高苎麻的耐旱耐盐性。
Transgenic technology is probably the best way to improve crops when traditional breeding is not feasible. Ramie is one such plant because the heterozygote complicates breeding efforts. This study is the first to report the successful production of transgenic ramie plants which is tolerant to drought and salt stress. In this study, the stress responsive gene SNAC1 (STRESS-RESPONSIVE NAC 1) was first introduced into ramie by Agrobacterium-mediated transformation which was using leaf midribs as explants. The overexpression of SNAC1 significantly enhanced drought and salt tolerance at the seedling and fiber maturation stages, via observation of phenotype and yield between transgenic plants and wild-type ones. SNAC1-overexpressing ramie plants also showed significantly improved tolerance to both drought and salt stresses at the rapid-growth stage. The transgenic plants had higher photosynthesis rates and relative water contents compared to the wild-type plants during the rapid-growth stage under drought and salt stress. Our study indicates that ramie's tolerance to drought and salt stress can be improved through genetic engineering.