Hormonally active nontransformed human ovarian cell culture from oophorectomy specimens: methods of development and initial characterization.
Hormonally active nontransformed human ovarian cell culture from oophorectomy specimens: methods of development and initial characterization.
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来自卵巢切除标本的激素活性非转化人卵巢细胞培养物：开发方法和初步表征。
DOI：
10.1159/000089349
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Poretsky,Leonid
中科院分区：
文献类型：
--
作者：
Seto-Young,Donna;Leonardi,Olga;Park,Alice;Holcomb,Kevin;Salehi,Marzieh;Chang,Peter;Yih,Melissa;Rosenwaks,Zev;Poretsky,Leonid
We repeatedly established a nontransformed steroidogenically active human ovarian cell culture derived from oophorectomy specimens. The cells maintained steroidogenic activity for 3–5 passages (6–8 weeks) and responded to stimulation by insulin and gonadotropin. With pregnenolone as substrate, LH stimulated progesterone production up to 124% and FSH up to 121%. Insulin alone stimulated progesterone production up to 135%, in the presence of LH up to 191%, and in the presence of FSH up to 170%. With dehydroisoandrosterone (DHA) as substrate, insulin alone stimulated testosterone production up to 117%, and in the presence of LH (but not FSH) up to 125%. With androstenedione as substrate, insulin alone stimulated estradiol production up to 133%, FSH alone up to 188%, and LH with insulin up to 217%. With progesterone as substrate and in the presence of LH (but not FSH), 17-α-hydroxylase activity was stimulated up to 131%. With DHA as substrate and in the presence of LH, 3-β-hydroxysteroid dehydrogenase (3-β-HSD) activity was stimulated up to 139%. With androstenedione as substrate, insulin alone stimulated aromatase activity up to 202%, LH up to 208%, and FSH up to 251%. Under the same conditions, in the presence of LH and insulin, aromatase activity was stimulated up to 342%, and in the presence of FSH and insulin, up to 318%. With testosterone as substrate, insulin alone stimulated aromatase activity up to 122%. With testosterone as substrate, in the presence of LH and insulin, aromatase activity was stimulated up to 136%, and in the presence of FSH and insulin, up to 156%. Immunocytochemistry studies directly confirmed presence of aromatase and 3-β-HSD in these cultured cells. We conclude that a steroidogenically active nontransformed long-term human ovarian cell culture can be repeatedly established from oophorectomy specimens, providing uninterrupted supply of cultured human ovarian cells for a variety of studies of ovarian physiology.
