Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids: a method applicable in humans
Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids: a method applicable in humans
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DOI：
10.1152/ajpendo.00579.2012
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发表时间：
2013-04-01
影响因子：
5.1
通讯作者：
Matthews, Dwight E.
中科院分区：
文献类型：
--
作者：
Holm, Lars;O'Rourke, Bruce;Matthews, Dwight E.
[10]霍尔姆L，O 'Rourke B，Ebenstein D，Toth MJ，Bechshoeft R，Holstein-Rathlou N，Kjaer M，马修斯DE.通过蛋白质结合示踪剂标记氨基酸的消失测定体内稳态蛋白质分解速率：一种适用于人体的方法。Am J Physiol Endocrinol Metab 304：E895-E907，2013.首次发表于2013年2月19日; doi：10.1152/ajpendo.00579.2012.-开发了一种测定单个蛋白质中蛋白质分解速率的方法，并在大鼠中进行了测试，并在人体中进行了确认，方法是使用氧化氘给药并将氘掺入丙氨酸，丙氨酸随后掺入体内蛋白质。从蛋白质中氘代丙氨酸的消失速率测定蛋白质的部分分解速率。使用指数衰减计算蛋白质中氘代丙氨酸的消失速率，给出蛋白质的部分分解速率（FBR）。这种蛋白质特异性FBR方法的适用性适用于人类体内实验。氧化氘给药的标记期取决于目标蛋白的周转率。
Holm L, O'Rourke B, Ebenstein D, Toth MJ, Bechshoeft R, Holstein-Rathlou N, Kjaer M, Matthews DE. Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids: a method applicable in humans. Am J Physiol Endocrinol Metab 304: E895-E907, 2013. First published February 19, 2013; doi:10.1152/ajpendo.00579.2012.-A method to determine the rate of protein breakdown in individual proteins was developed and tested in rats and confirmed in humans, using administration of deuterium oxide and incorporation of the deuterium into alanine that was subsequently incorporated into body proteins. Measurement of the fractional breakdown rate of proteins was determined from the rate of disappearance of deuterated alanine from the proteins. The rate of disappearance of deuterated alanine from the proteins was calculated using an exponential decay, giving the fractional breakdown rate (FBR) of the proteins. The applicability of this protein-specific FBR approach is suitable for human in vivo experimentation. The labeling period of deuterium oxide administration is dependent on the turnover rate of the protein of interest.