Cofilin/ADF is required for retinal elongation and morphogenesis of the Drosophila rhabdomere
Cofilin/ADF is required for retinal elongation and morphogenesis of the Drosophila rhabdomere
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DOI：
10.1016/j.ydbio.2008.03.006
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发表时间：
2008-06-01
影响因子：
2.7
通讯作者：
Laski, Frank A.
中科院分区：
文献类型：
--
作者：
Pham, Hung;Yu, Hui;Laski, Frank A.
果蝇光感受器在蛹发育过程中发生了明显的形态变化。这些变化包括视网膜细胞体的五倍伸长和横纹肌的形态发生，横纹肌是细胞的感光结构。在这里，我们表明，twinstar（TSR），其中编码果蝇cofilin/ADF（肌动蛋白解聚因子），这两个过程是必需的。在tsr突变体中，视网膜比正常人短，这是视网膜缺乏伸长的结果。此外，在强tsr突变体中，横纹肌结构紊乱，微绒毛短，偶尔散开。在一个中间型tsr突变体中，杆粒不是紊乱的，而是比正常结构更宽。还发现连接感光细胞彼此的粘附连接比正常情况下更宽。我们建议，并提供数据支持，这些广泛的横纹肌和adherens连接是继发性事件所造成的抑制视网膜伸长。这些结果提供了深入了解肌动蛋白细胞骨架的功能在果蝇眼睛的形态发生。(C)2008年爱思唯尔公司All rights reserved.
Drosophila photoreceptors undergo marked changes in their morphology during pupal development. These changes include a five-fold elongation of the retinal cell body and the morphogenesis of the rhabdomere, the light sensing structure of the cell. Here we show that twinstar (tsr), which encodes Drosophila cofilin/ADF (actin-depolymerizing factor), is required for both of these processes. In tsr mutants, the retina is shorter than normal, the result of a lack of retinal elongation. In addition, in a strong tsr mutant, the rhabdomere structure is disorganized and the microvilli are short and occasionally unraveled. In an intermediate tsr mutant, the rhabdomeres are not disorganized but have a wider than normal structure. The adherens junctions connecting photoreceptor cells to each other are also found to be wider than normal. We propose, and provide data supporting, that these wide rhabdomeres and adherens junctions are secondary events caused by the inhibition of retinal elongation. These results provide insight into the functions of the actin cytoskeleton during morphogenesis of the Drosophila eye. (C) 2008 Elsevier Inc. All rights reserved.