Domain structure elucidation of human decorin glycosaminoglycans
Domain structure elucidation of human decorin glycosaminoglycans
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DOI：
10.1042/bj20100788
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发表时间：
2010-10-15
影响因子：
4.1
通讯作者：
Linhardt, Robert J.
中科院分区：
文献类型：
--
作者：
Laremore, Tatiana N.;Ly, Mellisa;Linhardt, Robert J.
GAG的结构使用完整链分析和结构域组成分析的组合来检查重组表达的核心蛋白聚糖蛋白聚糖的（糖胺聚糖）链。GAG具有22 kDa的数均分子量，如通过PAGE NMR光谱分析使用两个-二维相关光谱表明，核心蛋白聚糖肽聚糖中葡萄糖醛酸与艾杜糖醛酸的比例为5：1 GAG结构域终止通过PAGE分析用高度特异性的内溶和外溶裂解酶酶解核心蛋白聚糖GAG获得的特异性二糖，并进一步用酶解聚。用液相色谱-质谱联用和光度检测法获得了该结构域的二糖组成谱，并与多糖的二糖组成谱进行了比较。通过二糖组成谱获得的信息与NMR和PAGE数据相结合，构建了核心蛋白聚糖GAG序列基序图谱。
The structure of the GAG (glycosaminoglycan) chain of recombinantly expressed decorin proteoglycan was examined using a combination of intact-chain analysis and domain compositional analysis The GAG had a number-average molecular mass of 22 kDa as determined by PAGE NMR spectroscopic analysis using two-dimensional correlation spectroscopy indicated that the ratio of glucuronic acid to iduronic acid in decorin peptidoglycan was 5 to I GAG domains terminated with a specific disaccharide obtained by enzymatic degradation of decorin GAG with highly specific endolytic and exolytic lyases were analysed by PAGE and further depolymerized with the enzymes. The disaccharide compositional profiles of the resulting domains were obtained using LC with mass spectrometric and photometric detection and compared with that of the polysaccharide The information obtained through the disaccharide compositional profiling was combined with the NMR and PAGE data to construct a map of the decorin GAG sequence motifs