Comparative analysis of genome sequences of three isolates of Orf virus reveals unexpected sequence variation
Comparative analysis of genome sequences of three isolates of Orf virus reveals unexpected sequence variation
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DOI：
10.1016/j.virusres.2005.09.011
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发表时间：
2006-03-01
期刊：
影响因子：
5
通讯作者：
Fleming, SB
中科院分区：
文献类型：
--
作者：
Mercer, AA;Norihito, UA;Fleming, SB
口蹄疫病毒（ORFV）是副痘病毒属的型种。在这里，我们展示了研究最充分的ORFV分离株NZ2的基因组序列。NZ2基因组长度为138 kbp，包含132个假定基因，其中88个存在于所有分析的脊索虫病毒中。将NZ2基因组与其他2个ORFV全测序分离株的基因组进行比较，发现3个基因组均携带132个基因，但分离株之间存在大量基因序列差异，其中9个分离株间氨基酸序列同源性仅为38-79%。每个基因组平均有64%的G + C，但在基因组的特定区域内，每个基因组都有明显的偏离平均值的模式。在另一种副痘病毒的基因组中也观察到相同的变异模式，这显然不同于在所有其他属的脊灰病毒中看到的G + C含量的统一模式。三株口蹄疫病毒分离株基因组序列的可用性使我们能够更准确地评估可能的编码区域，从而修改24 Genes的已发表数据，并预测两个以前未被识别的基因。(c) 2005 Elsevier b.v.。版权所有。
Orf virus (ORFV) is the type species of the Parapoxvirus genus. Here, we present the genomic sequence of the most well studied ORFV isolate, strain NZ2. The NZ2 genome is 138 kbp and contains 132 Putative genes, 88 of which are present in all analyzed chordopoxviruses. Comparison of the NZ2 genome with the genomes of 2 other fully sequenced isolates of ORFV revealed that all 3 genomes carry each of the 132 genes, but there are substantial sequence variations between isolates in a significant number of genes, including 9 with inter-isolate amino acid sequence identity of only 38-79%. Each genome has an average of 64% G + C but each has a distinctive pattern of substantial deviation from the average within particular regions of the genome. The same pattern of variation was also seen in the genome of another parapoxvirus species and was clearly unlike the uniform patterns of G + C content seen in all other genera of chordopoxviruses. The availability of genomic sequences of three orf virus isolates allowed us to more accurately assess likely coding regions and thereby revise published data for 24 Genes and to predict two previously unrecognized Genes. (c) 2005 Elsevier B.V.. All rights reserved.