CORRELATION OF THE STAGE OF CYTOPATHIC CHANGE WITH THE RELEASE OF POLIOMYELITIS VIRUS
CORRELATION OF THE STAGE OF CYTOPATHIC CHANGE WITH THE RELEASE OF POLIOMYELITIS VIRUS
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DOI：
10.1016/0042-6822(56)90034-4
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发表时间：
1956-01-01
期刊：
影响因子：
3.7
通讯作者：
DUNNEBACKE, TH
中科院分区：
文献类型：
--
作者：
DUNNEBACKE, TH
对猴肾、HeLa细胞和人胎细胞感染盖玻片培养物进行细胞学检查，发现每种类型的细胞发生了相似的细胞病变变化，并且这些变化对于所用的每种脊髓灰质炎病毒株都是相同的（I型，Mahoney; II型，MEF-1; III型，Saukett）。根据每个盖玻片的总细胞计数以及每个对照和感染培养物中每种细胞学类型的细胞数量，对细胞学观察结果进行定量评价。细胞学类型包括有丝分裂、非特异性变性、分裂间期和4个阶段的细胞病变：I期，早期核固缩，胞质无明显变化，II期，晚期核固缩，胞质收缩，胞质内有少量细胞核，胞质内有少量细胞核。III期，细胞中心形成淡染病变，核染色物质凝聚成小团块，细胞变圆; IV期，细胞解体。在猴肾细胞单层培养物上，通过空斑技术测定感染后细胞维持的培养基样品。细胞病变阶段与病毒释放时间的相关性表明，病毒释放发生在第III阶段之后数小时，第IV阶段之前。
Cyto-logical examination of infected cover slip cultures of monkey kidney, HeLa, and human fetal cells showed that each type underwent similar cytopathic changes and that these changes were identical for each of the strains of poliomyelitis used (Types I, Mahoney; II, MEF-1; III, Saukett). The cytological observations were given a quantitative evaluation in terms of the total cell count per cover slip and in term of the number of cells per cytological type in each control and infected culture. The cytological cell types included mitosis, nonspecific degeneration, interphase, and 4 arbitrarily designated stages of the cytopathic change: stage I, early nuclear pyknosis, no obvious cytoplasmic changes; stage II, advanced nuclear pyknosis with its displacement to one side of the cell, contraction of the cytoplasm; stage III, formation of a light-staining lesion in the center of the cell, nuclear staining material condensed into a small mass, cell rounded; stage IV, cell disintegration. Samples of the media in which the cells were maintained after infection were assayed by the plaque technique on monolayer cultures of monkey kidney cells. A correlation of the stage of cytopathic change with the time of virus release indicates that the release occurs several hours after stage III, and before stage IV.