Adaptation of the Griess reaction for detection of nitrite in human plasma
Adaptation of the Griess reaction for detection of nitrite in human plasma
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DOI：
10.1080/10715760400017327
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发表时间：
2004-11-01
影响因子：
3.3
通讯作者：
Rossi, R
中科院分区：
文献类型：
--
作者：
Giustarini, D;Dalle-Donne, I;Rossi, R
测定人血浆或血清中的亚硝酸盐最常用作一氧化氮（NO）产生的标志物。此外，最近有人建议，亚硝酸盐可以作为一种血管舒张剂在生理浓度的NO交付。因此，生物体液中亚硝酸盐的测定变得越来越重要。测量亚硝酸盐最常用的方法是基于与Griess试剂反应后获得的偶氮染料的分光光度分析。该方法在检测限和灵敏度方面存在一定的局限性，不适合血浆中亚硝酸盐的检测。我们已经发现了一些缺点，并修改了原来的程序，以克服这些问题。通过使用新开发的方法，我们测量了221 +/- 72 nM亚硝酸盐在健康供体的人血浆中。
The determination of nitrite in human plasma or serum has been most frequently used as a marker of nitric oxide ( NO) production. In addition, it has recently been suggested that nitrite could act as a vasodilating agent at physiological concentrations by NO delivery. Therefore, nitrite determination in biological fluids is becoming increasingly important. The most frequently used method to measure nitrite is based on the spectrophotometric analysis of the azo dye obtained after reaction with the Griess reagent. This method has some limitations regarding detection limit and sensitivity, thus resulting unsuitable for nitrite detection in plasma. We have identified some drawbacks and modified the original procedure to overcome these problems. By the use of the newly developed method, we measured 221 +/- 72 nM nitrite in human plasma from healthy donors.