Heterogeneous distribution of taste cells in facial and vagal nerve-innervated taste buds.
Heterogeneous distribution of taste cells in facial and vagal nerve-innervated taste buds.
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面部和迷走神经支配的味蕾中味觉细胞的异质分布。
DOI：
10.1016/j.neuroscience.2005.11.018
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Michel,WC
中科院分区：
文献类型：
--
作者：
Eram,M;Michel,WC
来自脊椎动物三条味觉神经的输入用于评估食物的营养质量。在某些物种中，这些颅神经被修改以完成额外的特定功能。例如，面神经支配分布在鲶鱼体表的味蕾，有助于食物搜索。生理学研究表明，这种口外味觉通路比口腔的舌咽或迷走神经系统对氨基酸更敏感。目前的研究旨在确定味觉细胞亚型的差异是否可能导致所观察到的敏感性差异。在2118个由面神经或迷走神经支配的斑点叉尾（Ictalurus punctatus）个体味觉细胞中，检测了5种低分子量代谢物L-丙氨酸、L-天冬氨酸、L-谷氨酸、GABA、牛磺酸和谷胱甘肽三肽的分布。这些细胞的代谢产物谱免疫细胞化学测定，并进行k-均值聚类算法。确定了15种具有定量不同代谢物共定位模式的细胞类别。所有，但一个小类的两个细胞被发现在面部和迷走神经支配的味蕾。四类（9%的总细胞）有高，两类（17%）有中间和其余九类（74%）有低水平的GABA免疫反应性。虽然代谢产物谱差异的功能意义仍有待确定，但味觉细胞类别在迷走神经和面神经支配的味蕾中分布不均匀，可能为先前报道的味觉敏感性差异提供解剖学基础。
Input from the three gustatory nerves of vertebrates is used to evaluate the nutritional quality of food. In some species, these cranial nerves are modified to accomplish additional specific functions. For example, the facial nerve innervated taste buds distributed over the body surface of catfish aid food search. Physiological studies indicate that this extra-oral taste pathway is more sensitive to amino acids than either the glossopharyngeal or vagal systems of the oral cavity. The current investigation seeks to determine if differences in taste cell subtypes might contribute to the observed differences in sensitivity. The distributions of five low molecular weight metabolites, l-alanine, l-aspartate, l-glutamate, GABA, taurine and the tripeptide glutathione, were examined in 2118 individual taste cells innervated by either the facial or vagal nerve of the channel catfish, Ictalurus punctatus. The metabolite profiles of these cells were determined immunocytochemically and subjected to a k-means clustering algorithm. Fifteen cell classes with quantitatively different patterns of metabolite co-localization were identified. All but one small class of two cells were found in both facial and vagal nerve-innervated taste buds. Four classes (9% of the total cells) had high, two classes (17%) had intermediate and the remaining nine classes (74%) had low levels of GABA immunoreactivity. While the functional significance of differences in metabolite profile remains to be determined, taste cell classes were not uniformly distributed across vagal and facial nerve innervated taste buds and may provide an anatomical basis for previously reported differences in gustatory sensitivity.