A mouse T cell product that preferentially enhances IgA production. II. Physicochemical characterization.
A mouse T cell product that preferentially enhances IgA production. II. Physicochemical characterization.
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一种优先增强 IgA 产生的小鼠 T 细胞产品。
DOI：
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发表时间：
1987
影响因子：
4.4
通讯作者：
Robert L. Coffman
中科院分区：
文献类型：
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作者：
M. Bond;B. Shrader;T. Mosmann;Robert L. Coffman
某些辅助性T细胞亚群在伴刀豆球蛋白A刺激后分泌特异性增强脂多糖刺激的B细胞产生IgG 1、IgE和伊加的因子。在以前的报告中，我们描述了一个因子从辅助T细胞系MB 2 -1，增强伊加的生产。IgA增强因子已从该细胞系的无血清上清液中纯化。纯化的淋巴因子是一个家族的微异质性多肽，推测修饰后的免疫。IgA增强因子具有天然m.w.在还原条件下，45，000至60，000的亚基在24，000至28，000之间。在Edman降解后，检测到与淋巴因子白细胞介素5相同的单个氨基末端序列。因此，IgA增强因子的活性是由相同的多肽介导的，所述多肽已被表征为II型B细胞生长因子、T细胞替代因子和嗜酸性粒细胞分化因子。
Certain subsets of helper T cells, following stimulation with concanavalin A, secrete factors that specifically enhance the production of IgG1, IgE, and IgA by lipopolysaccharide-stimulated B cells. In the previous report, we describe a factor from the helper T cell line MB2-1 which enhances IgA production. IgA-enhancing factor has been purified from serum-free supernatants of this cell line. The purified lymphokine is a family of microheterogeneous polypeptides presumably modified post-translationally. IgA-enhancing factor has a native m.w. of 45,000 to 60,000 with subunits of between 24,000 and 28,000 under reducing conditions. Upon Edman degradation, a single amino-terminal sequence is detected which is identical to that of the lymphokine interleukin 5. IgA-enhancing factor activity is thus mediated by the same polypeptide that has been characterized as type II B cell growth factor, T cell-replacing factor, and eosinophil-differentiation factor.