Abscisic acid plays critical role in ozone‐induced taxol production of Taxus chinensis suspension cell cultures
Abscisic acid plays critical role in ozone‐induced taxol production of Taxus chinensis suspension cell cultures
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DOI：
10.1002/btpr.660
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发表时间：
2011-09
影响因子：
2.9
通讯作者：
Maojun Xu;Haihong Jin;Jufang Dong;Ming Zhang;Xiangbin Xu;Ting Zhou
中科院分区：
文献类型：
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作者：
Maojun Xu;Haihong Jin;Jufang Dong;Ming Zhang;Xiangbin Xu;Ting Zhou
暴露于臭氧诱导的植物激素脱落酸（阿坝）的水平迅速增加，随后由紫杉醇生产的红豆杉悬浮细胞培养的增强。观察到的阿坝和紫杉醇的增加依赖于臭氧的浓度施加到T。chinensis细胞培养为了研究阿坝在臭氧诱导的紫杉醇产生中的作用，我们用阿坝生物合成抑制剂氟啶酮预处理细胞以消除臭氧触发的阿坝产生，并测定氟啶酮对臭氧诱导的紫杉醇产生的影响。结果表明，用氟啶酮预处理细胞不仅抑制了臭氧触发的阿坝产生，而且阻断了臭氧诱导的紫杉醇产生。此外，我们的数据表明，阿坝对紫杉醇生产的影响。chinensis细胞培养物具有剂量依赖性。有趣的是，氟啶酮对臭氧诱导的紫杉醇产生的抑制被外源性应用低剂量的阿坝逆转，尽管单独的低剂量阿坝处理对细胞的紫杉醇产生没有影响。总之，这些数据表明，臭氧是一种有效的激发子，用于提高植物细胞培养物的紫杉醇产量。此外，我们证明了阿坝在臭氧诱导的T.中国对虾悬浮细胞培养© 2011美国化学工程师学会生物技术。程序：2011
Exposure to ozone induced a rapid increase in the levels of the phytohormone abscisic acid (ABA) and sequentially followed by the enhancement of Taxol production in suspension cell cultures of Taxus chinensis. The observed increases in ABA and Taxol were dependent on the concentration of ozone applied to T. chinensis cell cultures. To examine the role of ABA in ozone‐induced Taxol production, we pretreated the cells with ABA biosynthesis inhibitor fluridone to abolish ozone‐triggered ABA generation and assayed the effect of fluridone on ozone‐induced Taxol production. The results showed that pretreatment of the cells with fluridone not only suppressed the ozone‐triggered ABA generation but also blocked the ozone‐induced Taxol production. Moreover, our data indicate that the effect of ABA on Taxol production of T. chinensis cell cultures is dose‐dependent. Interestingly, the suppression of fluridone on ozone‐induced Taxol production was reversed by exogenous application of low dose of ABA, although treatment of low dose ABA alone had no effect on Taxol production of the cells. Together, the data indicated that ozone was an efficient elicitor for improving Taxol production of plant cell cultures. Furthermore, we demonstrated that ABA played critical roles in ozone‐induced Taxol production of T. chinensis suspension cell cultures. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011