INDUCTION OF TRANSFORMING GROWTH FACTOR-ALPHA IN ACTIVATED HUMAN ALVEOLAR MACROPHAGES
INDUCTION OF TRANSFORMING GROWTH FACTOR-ALPHA IN ACTIVATED HUMAN ALVEOLAR MACROPHAGES
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DOI：
10.1016/0092-8674(88)90390-x
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发表时间：
1988-04-22
期刊：
影响因子：
64.5
通讯作者：
ROSS, R
中科院分区：
文献类型：
--
作者：
MADTES, DK;RAINES, EW;ROSS, R
在正常伤口修复和许多病理过程中观察到的早期单核细胞过滤先于上皮和结缔组织增殖反应，表明单核细胞/巨噬细胞可能是这些组织生长因子的重要来源。在培养中，活化的巨噬细胞分泌对成纤维细胞、平滑肌、内皮和上皮有活性的生长因子。该报告表明激活的人肺泡巨噬细胞表达转化生长因子-α的基因。（TGF-α）以诱导的方式，并将功能上和免疫学上与TGF-α相同的因子分泌到培养基中。TGF-α的两种不同分子种类。活性（约8500- 12，000和28，500道尔顿）。这些观察结果证实巨噬细胞是能够合成和分泌TGF-α的二倍体人细胞。因此，活化的巨噬细胞代表了促有丝分裂因子的细胞来源，该促有丝分裂因子在上皮细胞增殖和修复中可能是重要的。
The early monocyte filtration observed in normal wound repair and in a number of pathologic processes precedes the epithelial and connective tissue proliferative responses, suggesting that the monocyte/macrophage may be an important source of growth factors for these tissues. In culture, activated macrophages secrete growth factors active on fibroblasts, smooth muscle, endothelium, and epithelium. This report demonstrates that activated human alveolar macrophages express the gene for transforming growth factor-.alpha. (TGF-.alpha.) in an inducible manner and secrete a factor into the culture medium that is functionally and immunologically identical to TGF-.alpha.. Two different molecular species of TGF-.alpha. activity (approximately 8500-12,000 and 28,500 daltons) are identified in macrophage-conditioned medium. These observations establish the macrophage as a diploid human cell capable of synthesizing and secreting TGF-.alpha.. The activated macrophage therefore represents a cellular source of a mitogenic factor that is potentially important in epithelial proliferation and repair.