c-di-GMP Homeostasis Is Critical for Heterocyst Development in Anabaena sp. PCC 7120.
c-di-GMP Homeostasis Is Critical for Heterocyst Development in Anabaena sp. PCC 7120.
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c-di-GMP 稳态对于鱼腥藻异囊发育至关重要。
DOI：
10.3389/fmicb.2021.793336
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发表时间：
2021
影响因子：
5.2
通讯作者：
Zhang CC
中科院分区：
文献类型：
--
作者：
Huang M;Zhang JY;Zeng X;Zhang CC
c-二gmp是一种普遍存在的细菌信号，可调节多种生理过程。Anabaena PCC 7120 （Anabaena）是一种丝状蓝藻，在24小时的联合氮剥夺下，能够形成规则间隔的异囊进行固氮。水藻共有16个编码c-di-GMP代谢蛋白的基因，除all2874 （cdgS）基因缺失导致氮饥饿48h后异囊频率下降外，其他基因功能不详。我们在这里证明，在联合氮饥饿后，c-二gmp水平显著增加。通过灭活这16个基因，我们发现，缺失all1175 （cdgSH）导致异囊频率在氮降压后24小时增加。双突变体ΔcdgSHΔcdgS在调节杂种囊频率方面比单突变体具有加性效应，表明这两个基因在不同的时间点对杂种囊间隔起作用。生化和遗传学数据进一步表明，CdgSH和CdgS在异囊频率的建立或维持中的作用取决于它们对细胞内c-di-GMP水平的相反作用。最后，我们证明当c-di-GMP水平过高或过低时，异囊分化被完全抑制。综上所述，c-di-GMP水平的稳态对鱼腥鱼异囊分化具有重要意义。
c-di-GMP is a ubiquitous bacterial signal regulating various physiological process. Anabaena PCC 7120 (Anabaena) is a filamentous cyanobacterium able to form regularly-spaced heterocysts for nitrogen fixation, in response to combined-nitrogen deprivation in 24h. Anabaena possesses 16 genes encoding proteins for c-di-GMP metabolism, and their functions are poorly characterized, except all2874 (cdgS) whose deletion causes a decrease in heterocyst frequency 48h after nitrogen starvation. We demonstrated here that c-di-GMP levels increased significantly in Anabaena after combined-nitrogen starvation. By inactivating each of the 16 genes, we found that the deletion of all1175 (cdgSH) led to an increase of heterocyst frequency 24h after nitrogen stepdown. A double mutant ΔcdgSHΔcdgS had an additive effect over the single mutants in regulating heterocyst frequency, indicating that the two genes acted at different time points for heterocyst spacing. Biochemical and genetic data further showed that the functions of CdgSH and CdgS in the setup or maintenance of heterocyst frequency depended on their opposing effects on the intracellular levels of c-di-GMP. Finally, we demonstrated that heterocyst differentiation was completely inhibited when c-di-GMP levels became too high or too low. Together, these results indicate that the homeostasis of c-di-GMP level is important for heterocyst differentiation in Anabaena.
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DOI：
10.1073/pnas.1920143117
发表时间：
2020-04-28
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
64.5
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DOI：
10.1073/pnas.0511090103
发表时间：
2006-02-21
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
3.6
作者：
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