Regulation of the Cyanobacterial Circadian Clock by Electrochemically Controlled Extracellular Electron Transfer
Regulation of the Cyanobacterial Circadian Clock by Electrochemically Controlled Extracellular Electron Transfer
复制标题
DOI：
10.1002/anie.201309560
复制
发表时间：
2014-02-17
影响因子：
16.6
通讯作者：
Nakanishi, Shuji
中科院分区：
文献类型：
--
作者：
Lu, Yue;Nishio, Koichi;Nakanishi, Shuji
越来越多的人意识到生物钟与一系列物种的细胞内氧化还原状态密切相关。由于氧化还原状态是由氧化还原物质的交换决定的，电化学控制的细胞外电子转移（EC-EET）是一种细胞内电子与细胞外电极交换的过程，是一种很有前途的生物钟外部调节方法。在这里，我们以蓝藻长聚球菌PCC7942作为模型系统，讨论EC-EET是否可以调节生物钟。体内叶绿素荧光监测表明，EC-EET可以通过简单地改变电极电位来控制质体醌池的氧化还原状态。结果表明，即使在恒定光照条件下，也可以通过周期性调节的EC-EET模拟自然光/暗循环，成功地调控长叶蛇细胞的内源性生物钟。这是第一个通过电化学调节生物钟的例子。
There is growing awareness that circadian clocks are closely related to the intracellular redox state across a range of species. As the redox state is determined by the exchange of the redox species, electrochemically controlled extracellular electron transfer (EC-EET), a process in which intracellular electrons are exchanged with extracellular electrodes, is a promising approach for the external regulation of circadian clocks. Herein, we discuss whether the circadian clock can be regulated by EC-EET using the cyanobacterium Synechococcus elongatus PCC7942 as a model system. Invivo monitoring of chlorophyll fluorescence revealed that the redox state of the plastoquionone pool could be controlled with EC-EET by simply changing the electrode potential. As a result, the endogenous circadian clock of S. elongatus cells was successfully entrained through periodically modulated EC-EET by emulating the natural light/dark cycle, even under constant illumination conditions. This is the first example of regulating the biological clock by electrochemistry.