Computational Design of Membrane Proteins
Computational Design of Membrane Proteins
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DOI：
10.1016/j.str.2011.12.003
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发表时间：
2012-01-11
期刊：
影响因子：
5.7
通讯作者：
Saven, Jeffery G.
中科院分区：
文献类型：
--
作者：
Perez-Aguilar, Jose Manuel;Saven, Jeffery G.
膜蛋白参与各种各样的细胞过程，并且通常是细胞与细胞外分子的第一次相互作用的一部分。因此，这些蛋白质构成了大多数已知的药物靶标。膜蛋白是最难获得和表征的蛋白质之一，并且难以阐明其性质的基于结构的理解。尽管如此，膜蛋白的设计可以为我们对这些关键生物系统的理解提供严格的测试，并引入新的或有针对性的功能。计算设计方法在解决这些问题上特别有帮助，本文综述了最近的研究，定制膜蛋白显示特定的结构或功能，并探讨如何重新设计的膜蛋白被用来促进结构和功能的研究。
Membrane proteins are involved in a wide variety of cellular processes, and are typically part of the first interaction a cell has with extracellular molecules. As a result, these proteins comprise a majority of known drug targets. Membrane proteins are among the most difficult proteins to obtain and characterize, and a structure-based understanding of their properties can be difficult to elucidate. Notwithstanding, the design of membrane proteins can provide stringent tests of our understanding of these crucial biological systems, as well as introduce novel or targeted functionalities. Computational design methods have been particularly helpful in addressing these issues, and this review discusses recent studies that tailor membrane proteins to display specific structures or functions and examines how redesigned membrane proteins are being used to facilitate structural and functional studies.