N-terminal labeling of proteins by the Pictet-Spengler reaction
N-terminal labeling of proteins by the Pictet-Spengler reaction
复制标题
DOI：
10.1016/j.bmcl.2008.07.033
复制
发表时间：
2008-08-15
影响因子：
2.7
通讯作者：
Tachibana, Kazuo
中科院分区：
文献类型：
--
作者：
Sasaki, Tsubasa;Kodama, Koichiro;Tachibana, Kazuo
应用Pictet-Spengler反应对马心肌红蛋白的N-末端进行标记。这在以下两个步骤中进行：（1）通过转氨反应将N-末端甘氨酸残基转化为α-酮醛，以及（2）通过Pictet-Spengler反应将所得活化肌红蛋白与色胺类似物缩合。紫外（UV）/可见（维斯）吸收和圆二色性（CD）光谱数据显示，三级结构的肌红蛋白没有改变的Pictet-Spengler反应。(c)2008爱思唯尔有限公司保留所有权利。
The Pictet-Spengler reaction was applied to the N-terminal labeling of horse heart myoglobin. This was performed in the following two steps: (1) conversion of the N-terminal glycine residue to an alpha-keto aldehyde by a transamination reaction and (2) condensation of the resulting activated myoglobin with tryptamine analogues by the Pictet-Spengler reaction. Ultraviolet (UV)/visible (vis) absorption and circular dichroism (CD) spectral data revealed that the tertiary structure of myoglobin was not altered by the Pictet-Spengler reaction. (c) 2008 Elsevier Ltd. All rights reserved.