Effect of polydextrose on absorption of triglyceride and cholesterol in mesenteric lymph-fistula rats
Effect of polydextrose on absorption of triglyceride and cholesterol in mesenteric lymph-fistula rats
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DOI：
10.3181/00379727-215-44112
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发表时间：
1997-05-01
影响因子：
--
通讯作者：
Sakai, T
中科院分区：
文献类型：
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作者：
Ogata, S;Fujimoto, K;Sakai, T
在大多数实验设计中，水溶性膳食纤维对脂质吸收的抑制作用是通过降低血浆脂质浓度或增加粪脂产量来评价的。本研究的目的是评价水溶性多糖聚葡萄糖对淋巴瘘大鼠肠系膜淋巴脂质转运的急性影响，将大鼠的肠系膜淋巴管插管，并在十二指肠内插入输液管，复苏后，向十二指肠内注入含有放射性三油酸酯和油酸胆固醇的脂肪乳剂8小时。试验组用含5%或10%多聚葡萄糖的脂肪乳剂作为膳食纤维，输注结束时采集淋巴瘘标本，收集管腔内容物和粘膜。多聚葡萄糖组肠系膜淋巴流量在输注后减少，甘油三酯和胆固醇在肠腔内的残留量较多，这是由于脂类向淋巴的转运减少，在5%和10%的聚葡萄糖组中，这些影响是剂量依赖的。本研究表明，多聚葡萄糖延缓了三油油和胆固醇向肠系膜淋巴的转运。
In most experimental designs, the inhibitory effect of water-soluble dietary fibers on lipid absorption is evaluated by the decrease in plasma lipid concentration or the increase in fecal lipid output, The aim of this study was to evaluate the acute effect of a water-soluble polysaccharide, polydextrose, on lipid transport to the mesenteric lymph using lymph-fistula rats, The mesenteric lymph duct of rats was cannulated, and an infusion tube was introduced into the duodenum, After recovery, a lipid emulsion containing radioactive triolein and cholesteryl oleate was infused into the duodenum for 8 hr. The tested group was infused with the lipid emulsion containing 5% or 10% polydextrose as dietary fiber, Samples from the lymph-fistula were collected, and the luminal contents and mucosa were collected at the end of infusion. Lymph flow in the mesenteric lymph decreased in the polydextrose group after the infusion, The amounts of both triglyceride and cholesterol remaining in the lumen were greater in the polydextrose group, due to decreased transport of lipid into the lymph, These effects were dose dependent in the 5% and 10% polydextrose groups, The of this study indicate that polydextrose retarded the transport of triolein and cholesterol into the mesenteric lymph.