Association of Salmonella enterica serovar enteritidis YafD with resistance to chicken egg albumen
Association of Salmonella enterica serovar enteritidis YafD with resistance to chicken egg albumen
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DOI：
10.1128/iai.71.12.6734-6741.2003
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发表时间：
2003-12-01
影响因子：
3.1
通讯作者：
Riley, LW
中科院分区：
文献类型：
--
作者：
Lu, SW;Killoran, PB;Riley, LW
肠道沙门氏菌肠道血清型是工业化国家食源性疾病的主要原因。S.近几十年来，肠道血清型肠道菌感染大幅增加，S.肠道沙门氏菌血清型肠道沙门氏菌现在是美国沙门氏菌的主要血清型之一。S.肠道血清型肠道病与鸡壳蛋有关，因为大约80%的人类胃肠道疾病可追溯到受污染的蛋产品。当来自受感染母鸡生殖组织的细菌被包装到鸡蛋中并在不利的蛋清环境中持续存在时，鸡蛋被污染。因此，对卵蛋白的耐药性是沙门氏菌传播的一个重要方面。肠道血清型我们从S. enterica serovar enteritrium的过表达可使S.肠道血清型鼠伤寒沙门氏菌对蛋清的抗性增强，而在沙门氏菌中该基因的破坏。肠道血清型肠杆菌使生物体对蛋清更敏感。YafD与核酸外切酶-核酸内切酶-磷酸酶家族的成员同源，包括参与DNA修复的一些酶。此外，我们还发现，卵清蛋白具有核酸酶活性，并使用环状和线性DNA作为底物。我们认为，YafD提供了一个生存优势，S。结论：肠道沙门氏菌通过修复鸡蛋蛋白引起的DNA损伤，在鸡蛋中传播肠道沙门氏菌，可能是导致沙门氏菌流行病学关联的生物学决定因素之一。肠血清型肠球菌与蛋制品。
Salmonella enterica serovar Enteritidis is a major cause of food-borne diseases in industrialized countries. The incidence of S. enterica serovar Enteritidis infections has increased substantially in recent decades, and S. enterica serovar Enteritidis is now one of the leading serovars of Salmonella in the United States. A unique epidemiological characteristic of S. enterica serovar Enteritidis is its association with chicken shell eggs, since approximately 80% of all human gastrointestinal diseases can be traced to contaminated egg products. Eggs are contaminated when bacteria from reproductive tissues of infected hens are packaged into the eggs and persist inside the hostile egg albumen environment. Therefore, resistance to egg albumen is an important aspect in the transmission of S. enterica serovar Enteritidis. We identified a gene, yafD from S. enterica serovar Enteritidis, whose overexpression conferred upon S. enterica serovar Typhimurium enhanced resistance to egg albumen, while disruption of this gene in S. enterica serovar Enteritidis rendered the organism more susceptible to egg albumen. YafD is homologous to members of an exonuclease-endonuclease-phosphatase family, including some enzymes involved in DNA repair. Furthermore, we discovered that egg albumen has nuclease activities and uses both circular and linear DNA as substrates. We propose that YafD provides a survival advantage to S. enterica serovar Enteritidis in eggs by repairing DNA damage caused by egg albumen and that it may be one of the biologic determinants that contribute to the epidemiological association of S. enterica serovar Enteritidis with egg products.