MBNL1-RNA recognition: contributions of MBNL1 sequence and RNA conformation.
MBNL1-RNA recognition: contributions of MBNL1 sequence and RNA conformation.
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DOI：
10.1002/cbic.201100487
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发表时间：
2012-01-02
期刊：
影响因子：
3.2
通讯作者：
Baranger, Anne M.
中科院分区：
文献类型：
--
作者：
Fu, Yuan;Ramisetty, Sreenivasa Rao;Hussain, Nejmun;Baranger, Anne M.
Muscleblind-like proteins (MBNL) are RNA binding proteins that bind to the poly(CUG) and poly(CCUG) sequences that are the causative agents of myotonic dystrophy. It has been suggested that as a result of binding to the repeating RNA sequences, MBNL1 is abnormally expressed and translocated, which leads to many of the misregulated events in myotonic dystrophy. In this work, steady-state fluorescence quenching experiments suggest that MBNL1 alters the structure of helical RNA targets upon binding, which may explain the selectivity of MBNL1 for less structured RNA target sites. The removal of one pair of zinc fingers greatly impairs the binding affinity of MBNL1, which indicates that the two pairs of zinc fingers could possibly interact with RNA targets cooperatively. Alanine scanning mutagenesis suggested that the binding energy may be distributed across the protein. Overall, the results presented here suggest that small molecules that stabilize the helical structure of poly(CUG) and poly(CCUG) RNAs will inhibit the formation of complexes with MBNL1.
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能够在体外抑制（CUG）重复RNA-MBNL1相互作用的分子的动态组合选择：发现靶向肌发育症的铅化合物（DM1）。
影响因子：
15
作者：
Gareiss, Peter C.;Sobczak, Krzysztof;McNaughton, Brian R.;Palde, Prakash B.;Thornton, Charles A.;Miller, Benjamin L.
通讯作者：
Miller, Benjamin L.
影响因子：
2.9
作者：
GRAY, DM;LIU, JJ;ALLEN, FS
通讯作者：
ALLEN, FS
影响因子：
2.5
作者：
Hao, Minqi;Akrami, Kevan;Chen, Fabian
通讯作者：
Chen, Fabian
影响因子：
14.9
作者：
Kino, Yoshihiro;Washizu, Chika;Ishiura, Shoichi
通讯作者：
Ishiura, Shoichi
影响因子：
5.6
作者：
Hong, MK;Harbron, EJ;Musier-Forsyth, K
通讯作者：
Musier-Forsyth, K